No Correlation between Fagerstrom Test for Nicotine Dependence Scores to Lipopolysaccharide Marker in Thai Smokers

Parama Pratummas, Phakkharawat Sittiprapaporn, Chaiyavat Chaiyasut, Sasithorn Sirilun, Thamthiwat Nararatwanchai


Cigarette smoking is a combination of thousands of synthetic compounds that contribute straightforwardly or by implication to the addiction of cigarette smoking with inflammation, although a lot more may exist. Genome-wide association studies (GWAS) have unequivocally shown that variety in neuronal nicotinic acetylcholine receptor subunit α5 (CHRNA5) and in cytochrome P450 2A6 (CYP2A6) anticipated smoking substantialness, later period of smoking discontinuance.The relationship between the Fagerstrom Test for Nicotine Dependence and the Lipopolysaccharide marker in Thai smokers was proposed in this study.We investigated fourteen Thai smokers in Mahasarakham province who scored 4 or higher on the Fagerstrom Test for Nicotine Dependence. We provided them surveys about their personal information, administered the Fagerstrom Test for Nicotine Dependence, and continued the Lipopolysaccharide marker blood tests. The value of R in Pearson connection was 0.4438. Although it found a positive correlation, the relationship between Fagerstrom Test for Nicotine Dependence and Lipopolysaccharide level was weak due to no correlation. In addition, the value of R2, the coefficient of determination, was 0.197 (p=0.1119). Further studies are needed to investigate more the relationship between smoking and inflammation.


Smokers; cigarette smoking; nicotine dependence; lipopolysaccharide; inflammation.

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J. Gauthier, A.T. Vincent, S.J. Charette, and N. Derome, “A brief history of bioinformatics,†Brief Bioinform., vol. 20(6), pp. 1981-1996, 2019. doi: 10.1093/bib/bby063.

R.K. Tiwari, V. Sharma, R.K. Pandey, and S.S. Shukla, “Nicotine Addiction: Neurobiology and Mechanism,†J Pharmacopuncture., vol. 23(1), pp. 1-7, 2020.

K. Xu, B. Li, K.A. McGinnis, R. Vickers-Smith, C. Dao, N. Sun, R.L. Kember, H. Zhou, W.C. Becker, J. Gelernter, H.R. Kranzler, H. Zhao, A.C. Justice, and VA Million Veteran Program, “Genome-wide association study of smoking trajectory and meta-analysis of smoking status in 842,000 individuals,†Nat Commun., vol. 11(1), 5302, 2020.

L.J. Bierut, and R.F. Tyndale, “Preparing the Way: Exploiting Genomic Medicine to Stop Smoking,†Trends Mol Med., vol. 24(2), pp. 187-196, 2018.

J.I. de Granda-Orive, J.F. Pascual-Lledó, S. Asensio-Sánchez, S. Solano-Reina, M. da García-Rue, M.Ã. Martínez-Muñiz, L. Lázaro-Asegurado, D. Bujulbasich, R. Pendino, S. Luhning, I. Cienfuegos-AgustínI, and C.A. Jiménez-Ruiz, “Fagerström Test and Heaviness Smoking Index. Are they Interchangeable as a Dependence Test for Nicotine?,†Subst Use Misuse., vol. 55(2), pp. 200-208, 2020.

B. C. Quach, M. J. Bray, N. C. Gaddis, M. Liu, T. Palviainen, C. C. Minica, S. Zellers, R. Sherva, F. Aliev, M. Nothnagel, K. A. Young, J. A. Marks, H. Young, M. U. Carnes, Y. Guo, A. Waldrop, N. Y. A. Sey, M. T. Landi, D. W. McNeil, D. Drichel, L. A. Farrer, C. A. Markunas, J. M. Vink, J.-J. Hottenga, W. G. Iacono, H. R. Kranzler, N. L. Saccone, M. C. Neale, P. Madden, M. Rietschel, M. L. Marazita, M. McGue, H. Won, G. Winterer, R. Grucza, D. M. Dick, J. Gelernter, N. E. Caporaso, T. B. Baker, D. I. Boomsma, J. Kaprio, J. E. Hokanson, S. Vrieze, L. J. Bierut, E. O. Johnson, and D. B. Hancock, “Expanding the genetic architecture of nicotine dependence and its shared genetics with multiple traits,†Nat Commun., vol. 11, 5562, 2020.

S. Papoutsopoulou, J. Satsangi, B.J. Campbell, and C.S. Probert, “Review article: impact of cigarette smoking on intestinal inflammation—direct and indirect mechanisms,†Aliment Pharmacol Ther., vol. 51, pp. 1268– 1285, 2020.

L. Mazgaeen, and P. Gurung, “Recent advances in lipopolysaccharide recognition systems,†Int. J. Mol. Sci., vol. 21(2), 379, 2020.

B. Boonen, Y.A. Alpizar, A. Sanchez, A. López-Requena, T. Voets, and K. Talavera, “Differential effects of lipopolysaccharide on mouse sensory TRP channels,†Cell Calcium, vol. 73, pp. 72-81, 2018.

Y.A. Alpizar, B. Boonen, A. Sanchez, C. Jung, A. López-Requena, R. Naert, B. Steelant, K. Luyts, C. Plata, V.D. Vooght, J.A.J. Vanoirbeek, V.M. Meseguer, T. Voets, J.L. Alvarez, P.W. Hellings, P.H.M. Hoet, B. Nemery, M.A. Valverde, and K. Talavera, “TRPV4 activation triggers protective responses to bacterial lipopolysaccharides in airway epithelial cells,†Nat Commun., vol. 8, 1059, 2017.

L. Salhi, L. Seidel, A. Albert, and F. Lambert, “Fagerström test for nicotine dependence as an indicator in tobacco-related studies in periodontology,†J Periodontol., vol. 92(2), pp. 298-305, 2021.

N.L. Saccone, J.W. Baurley, A.W. Bergen, S.P. David, H.R. Elliott, M.G. Foreman, J. Kaprio, T.M. Piasecki, C.L. Relton, L. Zawertailo, L.J. Bierut, R.F. Tyndale, L.S. Chen, and Genetics and Treatment Networks of the Society for Research on Nicotine and Tobacco (SRNT), “The Value of Biosamples in Smoking Cessation Trials: A Review of Genetic, Metabolomic, and Epigenetic Findings,†Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco, vol. 20(4), pp. 403–413, 2018.

L.J. Bierut, “Langley Award for Basic Research on Nicotine and Tobacco: Bringing Precision Medicine to Smoking Cessation,†Nicotine Tob Res., vol. 22(2), pp. 147-151, 2020.

C.L. Mota, S. Mitri, C. Barata-Silva, and J.C. Moreira, “CHRNA5/CHRNA3 polymorphisms and tobacco smoking risk in a Brazilian population sample,†GSC Biol. Pharm. Sci., vol. 12(2), pp. 143–152, 2020.

Y.L. Strat, N. Ramoz, and P. Gorwood, “Evidence for the association of the nicotinic acetylcholine receptor CHRNA5/A3/B4 gene cluster and nicotine dependence in a young population of students,†French J. Psychiatry, vol. 6898(100), pp. S1-S182, 2020.

E.D. Coverstone, R.G. Bach, L. Chen, L.J. Bierut, A.Y. Li, P.A. Lenzini, H.C. O'Neill, J.A. Spertus, C.C. Sucharov, J.A. Stitzel, J.D. Schilling, and S. Cresci, “A novel genetic marker of decreased inflammation and improved survival after acute myocardial infarction,â€. Basic Res Cardiol., vol. 113(5), 38, 2018.

J. Dai, M. Huang, C.I. Amos, R.J. Hung, A. Tardon, A. Andrew, C. Chen, D.C. Christiani, D. Albanes, G. Rennert, J. Fan, G. Goodman, G. Liu, J.K. Field, K. Grankvist, L.A. Kiemeney, L. Le Marchand, M.B. Schabath, M. Johansson, M.C. Aldrich, M. Johansson, N. Caporaso, P. Lazarus, S. Lam, S.E. Bojesen, S. Arnold, M.T. Landi, A. Risch, H.E. Wichmann, H. Bickeboller, P. Brennan, S. Shete, O. Melander, H. Brunnstrom, S. Zienolddiny, P. Woll, V. Stevens, Z. Hu, and H. Shen, “Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk,†Int J Cancer., vol. 146(10), pp. 2855-2864, 2020. doi: 10.1002/ijc.32698.

A. Dutkowska, B. Szmyd, M. Kaszkowiak, and D. Daria Domańska-Senderowska, D. Pastuszak-Lewandoska, E. Brzeziańska-Lasota, J. Kordiak, and A. Antczak, “Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer,†Sci Rep., vol. 11, 5092, 2021.

R.L. Papke, D.H. Brunzell, and M. De Biasi, “Cholinergic Receptors and Addiction. Curr Top Behav Neurosci., vol. 45, pp. 123-151, 2020.

A. Jackson, D. Bagdas, P.P. Muldoon, A.H. Lichtman, F.I. Carroll, M. Greenwald, M.F. Miles, and M.I. Damaj, “In vivo interactions between α7 nicotinic acetylcholine receptor and nuclear peroxisome proliferator-activated receptor-α: Implication for nicotine dependence. Neuropharmacology, vol. 15(118), pp. 38-45, 2017.

R.M. Schuster, G.N. Pachas, L. Stoeckel, C. Cather, M. Nadal, D. Mischoulon, D.A. Schoenfeld, H. Zhang, C. Ulysse, E.B. Dodds, S. Sobolewski, V. Hudziak, A. Hanly, M. Fava, and A.E. Evins, “Phase IIb Trial of an α7 Nicotinic Receptor Partial Agonist With and Without Nicotine Patch for Withdrawal-Associated Cognitive Deficits and Tobacco Abstinence,†J Clin Psychopharmacol., vol. 38(4), pp. 307-316, 2018.



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