The Application of Clay Pot for Moisture Reduction of Geniotrigona thoracica Stingless Bee Honey

Nashratul Shera Mohamad Ghazali, Yus Aniza Yusof, Chin Nyuk Ling, Siti Hajar Othman, Yanty Noorzianna Abdul Manaf, Syahrul Anis Hazwani Mohd Baroyi


Geniotrigona thoracica (G. thoracica) stingless bee honey contained high moisture that can easily lead to fermentation process. The high moisture content of stingless bee honey could lead to unwanted fermentation and crystallization form. Hence, it will affect the quality of honey in terms of acidic taste and undesirable appearance.  To make sure stingless bee honey meets the standard set by IHC, some methods have been carried out to reduce its moisture content.  This study investigates the moisture reduction during storage of G. thoracica stingless bee honey in a clay pot. The G. thoracica honeys were stored in clay pot with the inner volume of 75.4cm3 and glass bottle (as control) at 25˚C for 21 days. Physicochemical analysis of honey such as moisture content, total soluble solid, water activity, viscosity, pH, free acidity, electrical conductivity, colour (L*, a* and b*) and colour intensity were investigated during storage. The result obtained indicated that the honey stored in a clay pot was significantly reduced the moisture content from 28.03% to 16.51%. The water activity (aw) of honey in a clay pot was also reduced from 0.779 to 0.601. The free acidity of honey stored in a glass bottle increased significantly compared to honey stored in a clay pot, which was initially 106.7meq/kg to 146.3meq/kg and 121.7meq/kg, respectively. In conclusion, the application of clay pot as a storage medium was capable to reduce water content and preserved the quality of honey from G. thoracica.


physicochemical; moisture; honey; Geniotrigona thoracica; clay.

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P. V. Rao, K. T. Krishnan, N. Salleh, and S.H. Gan, “Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative reviewâ€, Brazilian Journal of Pharmacognosy, vol. 26, pp. 657–664, 2016.

M. A. Abd Jalil, A. R. Kasmuri, and H. Hadi, “Stingless bee honey, the natural wound healer: a reviewâ€, Skin Pharmacology and Physiology, vol. 30, pp. 66–75, 2017.

M. Halcroft, R. Spooner-Hart, and L. A. Dollin, “Australian Stingless Bees,†in Pot Honey: A Legacy of Stingless Bees, P. Vit, S. R. M. Pedro, D. W. Roubi, Eds. New York, NY, USA: Springer, 2013.

C. D. Michener, “The Meliponini,†in Pot Honey: A Legacy of Stingless Bees, P. Vit, S. R. M. Pedro, D. W. Roubi, Eds. New York, NY, USA: Springer, 2013

B. Chuttong, Y. Chanbang, K. Sringarm, and M. Burgett, “Physicochemical profiles of stingless bee (Apidae: Meliponini ) honey from South East Asia ( Thailand ),†Food Chemistry, vol. 192, pp. 149–155, 2016b.

A. Nordin, H. Q. A. V. Sainik, S. R. Chowdhury, A. Saim, and R. B. H. Idrus, “Physicochemical properties of stingless bee honey from around the globe: A comprehensive reviewâ€, Journal of Food Composition and Analysis, vol. 73, pp. 91–102, 2018.

W. A. Azmi, R. Ghazi, and I. S. Nasharuddin, “Morphological, Nest Architecture and Colony Characteristics of Stingless Bees (Hymenoptera; Apidae; Meliponini) from Tasik Kenyir, Terengganuâ€, Greater Kenyir Landscapes; Springer: Cham, pp.111–121, 2019.

N. F. M. Saufi, and K. Thevan, “Characterization of nest structure and foraging activity of stingless bee, Geniotrigona thoracica (hymenopetra: apidae; meliponini)â€, Jurnal Teknologi, vol. 77(33), pp. 69–74, 2015.

F. C. Biluca, F. Braghini, L. V. Gonzaga, A. Carolina, O. Costa, and R. Fett, “Physicochemical profiles, minerals and bioactive compounds of stingless bee honey ( Meliponinae )â€, Journal of Food Composition and Analysis, vol. 50, pp. 61–69, 2016.

P. Vit, S. Bogdanov, and V. Kilchenmann, “Composition of Venezuelan honeys from stingless bees (Apidae: Meliponinae) and Apis mellifera Lâ€, Apidologie, vol. 25, pp. 278–288, 1994.

Bijlsma, L., Bruijn, L. L. M. de, Martens, E. P., & Sommeijer, M. J. (2006). Water content of stingless bee honeys (Apidae, Meliponini): interspecific variation and comparison with honey of Apis mellifera*. Apidologie, 37, 480–486.

A. J. Rodríguez-Malavera, C. Rasmussen, M. G. Gutiérrez, F. Gild, B. Nieves, and P. Vit,“Properties of honey from ten species of Peruvian stingless beesâ€, Natural Product Communications, vol. 4 (9), pp. 1221–1226, 2009.

K. Y. Chong, N. L. Chin, and Y. A. Yusof, “Thermosonication and optimization of stingless bee honey processingâ€, Food Science and Technology International, vol.23(7), pp. 608–622, 2017.

International Honey Commission, 2009. Harmonised Methods of the International Honey Commission, 63 pp. Swiss Bee Research Centre, Bern: FAM, Liebefeld

R. Subramanian, H. U. Hebbar, and N. K. Rastogi, “Processing of honey: a reviewâ€, International Journal of Food Properties, vol. 10, pp. 127–143, 2007.

D. Rhodes, Clay and Glazes for Potter. Philadelphia: Chilton Book Company, 1957.

Y. Dah-traoré, L. Zerbo, M. Seynou, and R. Ouedraogo, “Mechanical, Microstructural and Mineralogical Analyses of Porous Clay Pots Elaborated with Rice Husksâ€, Journal of Minerals and Materials Characterization and Engineering, vol. 6, pp. 257–270, 2018.

A. J. Varkey, and M. Dlamini, “Point-of-use water purification using clay pot water filters and copper meshâ€, Water SA, vol. 38(5), pp. 721–726, 2012.

W. Wongsakoonkan, T. Prechthai, and K. Tantrakarnapa, “Suitable types and constituent ratios for clay-pot water filters to improve the physical and bacteriological quality of drinking waterâ€, EnvironmentAsia, vol. 7(2), pp. 117–123, 2014.

G. Sesta, and L. Lusco, “Refractometric determination of water content in royal jelly*â€, Apidologie, vol. 39, 2008.

Association of Official Analytical Chemist (AOAC). In: W. Horwitz (Ed.) (18. ed.). Gaithersburg, MD, USA: Association of Official Analytical Chemists, 2005.

J. M. B. de Sousa, E. L. de Souza, G. Marques, M. de T. Benassi, B. Gullón, M. M. Pintado, and M. Magnani, “Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid regionâ€, LWT - Food Science and Technology, vol. 65, pp. 645–651, 2016.

A. Abdulkhaliq, and K. M. Swaileh, “Physico-chemical properties of multi-floral honey from the West Bank , Palestineâ€, International Journal of Food Properties, vol. 20(2), pp. 447–454, 2017.

M. F. Abu Bakar, S. B. Sanusi, F. I. Abu Bakar, O. J. Cong, and Z. Mian, “Physicochemical and antioxidant potential of raw unprocessed honey from Malaysian stingless beesâ€, Pakistan Journal of Nutrition, vol. 16(11), pp. 888–894, 2017.

M. P. Mathai, and A. Simon, “Water diffusion through pottery discs of varying porosityâ€, Journal of Tropical Agriculture, vol. 42(1–2), pp. 63–65, 2004.

S. Shamsudin, J. Selamat, M. Sanny, S. Abd, N. N. Jambari, Z. Mian, and A. Khatib, “Influence of origins and bee species on physicochemical, antioxidant properties and botanical discrimination of stingless bee honeyâ€, International Journal of Food Properties, vol. 22(1), 239–264, 2019.

C. A. Fuenmayor, A. C. Díaz-Moreno, C. M. Zuluaga-Domínguez, M. C. Quicazán, “Honey of Colombian stingless bees: nutritional characteristics and physicochemical quality indicatorsâ€, in Pot Honey: A Legacy of Stingless Bees, P. Vit, S. R. M. Pedro, D. W. Roubi, Eds. New York, NY, USA: Springer, 2013, pp. 3-17.

S. P. Kek, N. L. Chin, Y. A. Yusof, S. W. Tan, and L. S. Chua, “Classification of entomological origin of honey based on its physicochemical and antioxidant propertiesâ€, International Journal of Food Properties, pp. 1–16, 2017.

S. Yanniotis, S. Skaltsi, and S. Karaburnioti, “Effect of moisture content on the viscosity of honey at different temperaturesâ€, Journal of Food Engineering, vol. 72, pp. 372–377, 2006.

S. K. Yap, N. L. Chin, Y. A. Yusof, and K. Y. Chong, “Quality characteristics of dehydrated raw Kelulutâ€, International Journal of Food Properties, vol. 22(1), pp. 556–571, 2019.

P. M. da Silva, C. Gauche, L. V. Gonzaga, A. C. O. Costa, and R. Fett, “Honey: chemical composition, stability and authenticityâ€, Food Chemistry, vol. 196, pp. 309–323, 2016.

L. P. Oddo, T. A. Heard, A. Rodríguez-malaver, R. A. Pérez, M. Fernández-muiño, M. T. Sancho, G. Sesta, L. Lusco, P. Vit, “Composition and Antioxidant Activity of Trigona carbonaria Honey from Australiaâ€, Journal of Medicinal Food, vol. 11(4), pp. 789–794, 2008.

F. Tornuk, S. Karaman, I. Ozturk, O. S. Tokera, B. Tastemur, O. Sagdic, M. Dogan, and A. Kayacier, “Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profileâ€, Industrial Crops & Products, vol. 46, pp. 124–131, 2013.

A. Terrab, A. F. Recamales, D. Hernanz, and F. J. Heredia, “Characterisation of Spanish thyme honeys by their physicochemical characteristics and mineral contentsâ€, Food Chemistry, vol. 88, pp. 537–542, 2004.

M. Solayman, A. Islam, S. Paul, Y. Ali, M. I. Khalil, N. Alam, and S. H. Gan, “Physicochemical properties, minerals , trace elements , and heavy metals in honey of different origins : a comprehensive reviewâ€, Comprehensive Reviews in Food Science and Food Safety, vol. 15, pp. 219–233, 2016.

B. Chuttong, Y. Chanbang, K. Sringarm, and M. Burgett, “Effects of long term storage on stingless bee (Hymenoptera: Apidae: Meliponini) honeyâ€, Journal of Apicultural Research,2016a.

M. N. Lani, A. H. Zainudin, S. B. Abdul Razak, A. Mansor, and Z. Hassan, “Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperatureâ€, Malaysian Applied Biology, vol. 46(3), pp. 89–96, 2017.

L. B. de Almeida-Muradian, K. M. Stramm, A. Horita, O. M. Barth, A. da S. de Freitas, and L. M. Estevinho, “Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis melliferaâ€, International Journal of Food Science and Technology, vol. 48, pp. 1698–1706, 2013.

Codex Alimentarius Commission (2001). Alinorm 41/10: Revised standard for honey, Alinorm 1, 19–26.

E. Olga, F.-G. María, and S. M. Carmen, “Differentiation of Blossom Honey and Honeydew Honey from Northwest Spainâ€, Agriculture, pp. 25–37, 2012.

A. S. Alqarni, A. A. Owayss, A. A. Mahmoud, and M. A. Hannan, “Mineral content and physical properties of local and imported honeys in Saudi Arabiaâ€, Journal of Saudi Chemical Society, vol. 18, pp. 618–625, 2014.

USDA. 1985. United states standards for grades of extracted honey. May 23. Washington, DC: USDA, Agricultural Marketing Service

L. D. Sant’Ana, A. B. B. Ferreira, M. C. A. Lorenzon, R. L. L. Berbara, and R. N. Castro, “Correlation of total phenolic and flavonoid contents of Brazilian honeys with colour and antioxidant capacityâ€, International Journal of Food Properties, vol. 17, pp. 65–76, 2014.



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