Effects of Leucaena leucocephala Supplementation to Total Mixed Ration Based on Ammoniated Rice Straw Onfiber Digestibility and Rumen Fermentation Characteristics in vitro

Rusmana Wijaya Setia Ningrat, Mardiati Zain, - Erpomen, Ezi Masdia Putri, Malik Makmur

Abstract


Legume is a feed ingredient that contains high protein for ruminants. The tannin content in legumes can provide a bypass protein for ruminants. This study was done to find out the effect of legume supplementation (Leucaena leucocephala) on fiber digestibility, characteristic rumen, protozoa population and methane production from total mixed ration (TMR) based on ammoniated rice straw. The study was conducted in vitro using a randomized block design with 3 treatments and 5 replications. The treatment was A. 40% ammoniated rice straw + 60% concentrate, B. 40% ammoniated rice straw + 50% concentrate + 10% L. leucocephala, C. 40% ammoniated rice straw + 40% concentrate +10, + 20% L.leucocephala.The results showed that the addition of L. leucocephala increased digestibility of NDF, ADF, and Cellulose,  RUP (rumen undegradable protein) and reduced protozoa population and methane production (p <0.05). Increased doses of L.leucocephala up to 20% reduce fiber digestibility of feed substances compared to a dose of 10% but are still higher than controls. The results of this study concluded that L.leucocephala supplementation in TMR based on ammoniated rice straw in vitro improved digestibility, fermentability, and reduced methane gas production. Supplementation of 10% and 20% L. leucocephala needs further research, to see the effect on livestock in vivo.

Keywords


digestibility; Leucaena leucocephala; completed feed; ammoniated rice straw.

Full Text:

PDF

References


Sutardi, T., 1991. Aspek Nutrisi Sapi Bali. In Prosiding Seminar Pengembangan Sapi Bali. Universitas Hasanudin. Ujung Pandang.

Panjaitan, T.S., 2002. Mengenal Potensi Lamtoro Hibrida F2 sebagai Sumber Pakan Hijauan Ternak.BPTP Nusa Tenggara Barat.

Tilley, J.M. and Terry, R.A. 1963. A two stage technique for in vitro digestion of forage crops. Journal of British Grassland Society, 18: 104-111

AOAC, 2007.Official Method of Analysis.Association of Official Analytical Chemist, Washington D.C.

Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3579 – 3583.

General Laboratory Prosedure, 1996. Departement of Dairy Science, University of Wisconsin.

Conway, E.J. and E. O’Malley, 1942. Microdiffusion: Ammonia and urea using bufferedabsorbents (revised methods for ranges greaterthan 10 μg N). Biochem. J., 36: 655-566.

Ogimoto, K. and S. Imai. 1981. Atlas of RumenMicrobiology. Japan Scientific Societies Press, Tokyo.

Makkar, H.P.S., O. P. Sharma, R.K.Dawra and S.S.Negi. Simple determinination of microbial protein in rumen liquor. 1981. J. Dairy Sci 65: 2170-2173.

Steel, R.G.D. and Torrie, J.H. 1980. Principles and Procedures of Statistics, A Biometrical Approach. McGraw Hill, New York.

Aregheore, E.M. 1999. Nutritive and antinutritive value of some tree legumes used in ruminant livestock nutrition in Pacific island countries. Journal of South Pacific Agriculture. 6: 50-61

Hariadi BT, Santoso B. 2010. Evaluation of tropical plants containing tannin on in-vitro methanogenesis and fermentation parameters using rumen fluid. J Sci Food Agri. 90:456–461.

Patra, A.K., 2010. Meta-analyses of effects of phytochemicalson digestibility and rumen fermentation characteristicsassociated with methanogenesis.J. Sci. Food. Agric., 90. p. 2700-2708.

Galindo, J., Marrero, Y., Ruiz, T., González, N., Díaz, A., Aldama, A., Moreira, O., Hernández, J., Torres V., Sarduy, L. 2009. Efecto de una mezcla múltiple de leguminosas herbáceas y Leucaenaleucocephala en la población microbiana y productos fermentativos del rumen de añojos mestizos de Cebú. Revista Cubana de Ciencia Agrícola. 43: 256-264

Jayanegara, A., N. Togtokhbayar, H. P. S.Makkar& K. Becker.2008. Tannins determined by various methods as predictors of methane production reduction potential of plants by an in vitro rumen fermentation system. Anim. Feed Sci. Technol. 150: 230- 237

Wina, E., Muetzel,S. and Becker, K. 2005. The Impact of Saponin-Containing PlantMaterials on RuminantProduction-A review: J Agric Food Chem. 19:53(21):8093-105.

Ningrat, RWS., Zain, M., Erpomen, and Suryani, H. 2016. Effects of Doses and Different Sources of Tannins on in vitro Ruminal Methane, Volatile Fatty Acids Production and on Bacteria and Protozoa Populations.Asian J. AnimSci 11: 47-53

Jayanegara, A., F. Leiber, and M. Kreuzer. 2012. Meta-analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. Journal of Animal Physiology and Animal Nutrition 96, 365-375.http://dx.doi.org/10.1111/j.1439-0396.2011.01172.x

Morgavi, D. P., E. Forano, C. Martin, and C. J. Newbold. 2010. Microbial ecosystem and methanogenesis in ruminantsAnimal. 4:1024-1036.http://dx.doi.org/10.1017/ S1751731110000546

Hristov, A. N., J. Oh, J. L. Firkins, J. Dijkstra, E. Kebreab, G. Waghorn, H. P. S. Makkar, A. T. Adesogan, W. Yang, C. Lee, & P. J. Gerber. 2013. Mitigation of methane and nitrous oxide emissions from animal operations: I. A re¬view of enteric methane mitigation options. J. Anim. Sci. 91:5045-5069. http://dx.doi.org/10.2527/jas.2013-6583

McSweeney, CS, Palmer B, McNeill DM and Krause DO.2001. Microbial interactions with tannins: nutritional consequences for ruminants. Animal Feed Science and Technology 91, 83–93.

Heni Suryani, M Zain, RWS Ningrat, N Jamarun. 2016. Supplementation of Direct Fed Microbial (DFM) on in vitro Fermentability and degradability of ammoniated palm frond. Pakistan Journal of Nutrition 15 (1): 89-94

Susanti, S., Chuzaemi, S dan Soebarinoto. 2001. Pengaruh pemberian konsentrat yang mengandung bungkil kedelai, biji kapuk terhadap kecernaan ransum, produk fermentasi dan jumlah protozoa rumen sapi perah Peranakan Friesian Holstein jantan. Thesis. Fakultas Peternakan Universitas Brawijaya, Malang.

Calsamiglia, S., Ferret, A., Reynolds, C., Kristensen,N., van Vuuren, A. 2010. Strategies for optimizing nitrogen use by ruminants. Animal. 4: 1184-1196.

Hungate, R. E. 1966. The Rumen Microbial Ecosystem. Elsvier Applied science. London and New York.

Chuzaemi, S. Dan J.V. Bruchem. 1990. Fisiologi Nutrisi Ruminansia. Universitas Brawijaya. Malang.

Leng, R.A., 1991. Application of Biotechnology toNutrition of Animal in Developing countries. FAOProduction and Health Paper.

Roffler RE and Satter LD 1975.Relationship between ruminal ammonia and non protein nitrogen utilization by ruminants. I. Development of a model for predicting non protein nitrogen utilization by cattle. Journal of Dairy Science 58, 1880-1888

Jayanegara, A., Goel, G., Makkar, H.P.S. & Becker, K.2010. Reduction in Methane Emissions from Ruminants by Plant Secondary Metabolites: Effects of Polyphenols and Saponins. IN N.E. Odongo, M. Garcia & G.J. Viljoen (eds), Sustainable Improvement of Animal Production and Health. Food and Agriculture Organization of the United Nations, Rome, 2010: 151–157

Patra, A., and Saxena, J. 2009. The effect and mode of action of saponins on the microbial populations and fermentation in the rumen and ruminant production. Nutrition Research Reviews 22(2):204-19

Gutierrez, J. 2007. Observations on bacterial feeding by the rumen ciliate Isotricha prostoma,†Journal Eukaryotic Microbiology, vol. 5, pp. 122-126.

Czerkawski, J.W. 1986. An Introduction to Rumen Studies. Robert Maxwell, M. C., Oxford, UK

Mc.Donald, P., R. A. Edwards, J. F. D. Greenhalgh, and C. A. Morgan. 2002. Animal Nutrition. 6th ed. Ashford Colour Press Ltd, Gosport. pp. 515-535.

Wolin, M.J., T.L Miller and C.S Stewart. 1997.Microbe-microbe Interactions. In: The RumenMicrobial Ecosystem. HOBSON, P.N. and C.SSTEWART (Ed.).

Bhatta, R., Y. Uyeno, K. Tajima, A. Takenaka, Y. Yabumoto, I. Nonaka, O. Enishi, and M. Kurihara. 2009. Difference in the nature of tannins on in vitro ruminal methane and volatile fatty acid production and on methanogenic archaea and protozoal populations. Journal of Dairy Science 92:5512-5522.http://dx.doi.org/10.3168/jds.2008-1441

Jayanegara, A., F. Leiber, and M. Kreuzer. 2012. Meta-analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. Journal of Animal Physiology and Animal Nutrition 96, 365-375.http://dx.doi.org/10.1111/j.1439-0396.2011.01172.x

Retnani Y, Kamesworo S, Khotidjah L, Saenab A. 2010. Pemanfaatan Wafer Limbah Sayuran Pasar Untuk Ternak Domba.Seminar Nasional Teknologi Peternakan dan Veteriner, 2010 Agustus 2-3; Bogor, Indonesia. Bogor (ID): Pusat Penelitian dan Pengembangan Peternakan. 503-510.

Tavendale, M.H., Meagher, L.P., Pacheco, D., Walker, N., Attwood, G.T. Sivakumaran, S. 2005. Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis, Animal Feed Science and Technology, 123/124: 403-419.

Makkar, H.P.S. 2003. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin rich feeds.Small Ruminant Research. 49: 241-256

Mueller-Harvey, I. 2006.Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture 86:2010-2037.http://dx.doi.org/10.1002/jsfa.2577.

Monforte-Briceño, G., Sandoval-Castro, C., Ramírez-Avilíes, L., Capetillo-Leal, M. 2005. Defaunating capacity of tropical fodder trees: Effects of polyethylene glycol and its relationship to in vitro gas production. Animal Feed Science and Technology. 123/124: 313-327.




DOI: http://dx.doi.org/10.18517/ijaseit.9.3.8009

Refbacks

  • There are currently no refbacks.



Published by INSIGHT - Indonesian Society for Knowledge and Human Development