Surface Modification of Microcporous of Polycaprolactone (PCL) Microcarrier to Improve Microcarrier Biocompatibility

Nurhusna Samsudin, Yumi Z. H Y. Hashim, Mohd A. Arifin, Hamzah M. Salleh

Abstract


Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in the same solvent used to dissolve raw PCL. The evaporation of solvent and sublimination of camphene during the process of emulsion solvent evaporation method, produce solidified porous microcarrier beads. The surface porous microcarriers beads were further modified to make it competent for cell attachment and proliferation. The surface of these microcarriers were modified with UV/O3 system to introduce functional group and charge on the surface. The treatment followed with the immobilization of halal gelatin on the surface to imorove the biocompatibility of the microcarrier. Porous microcarrier with optimal size distribution was successfully fabricated. The average pore size of 11.74±8.32 µm was obtained with the concentration of 20% (w/v) of camphene. The porous PCL microcarrier was further tailored with gelatin through surface treatment with UV/O3 system and gelatin immobilization and validation of its compatibility towards mammalian cell was tested with cultivation of green monkyer kidney cell (Vero) in the suspension culture. Vero cells attach and proliferatie well on the gelatin coated porous PCL microcarrier with maximum cell number  of 3.90 × 105 cells/ml as compared to cell proliferation on UV/O3 treated porous PCL microcarrier (1.83 × 105 cells/ml). The developed microcarrier may be potentially applicable as a cell delivery scaffold for cell tissue culture and tissue engineering application.


Keywords


Halal; porous microcarrier; UV/O3 treatment; microcarrier cell culture.

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References


S. J. Hong, H. S. Yu and H. W. Kim. “Tissue engineering polymeric microcarriers with macroporous morphology and bone-bioactive surfaceâ€. Macromol. Biosci., vol. 9, pp. 639–645, 2009.

U. S. Shin, J-H. Park, S-J. Hong, J-E. Yu and W-H. Kim. “Porous biomedical composite microspheres developed for cell delivering scaffold in bone regeneration.†Mater. Lette., vol. 64, pp. 2261–2264, 2010.

Q. Zhang, K. Tan, Z. Ye, Y. Zhang, W. Tan and M. Lang. “Preparation of open porous polycaprolactone microspheres and their applications as effective cell carriers in hydrogel system.†Mater. Sci. and Engin.C, vol. 32(8), pp. 2589–2595, 2012

J. Li, A. T-L. Lam, J. P. W. Toh, S. Reuveny, S. K-H. Oh and W. K. Birch. “Tunable volumetric density and porous structure of spherical poly-ε-caprolactone microcarriers, as applied in human mesenchymal stem cell expansion.†Langmuir, vol. 33, pp. 3068-307, 2017.

B. K. Kim, S. J. Hwang, J. B. Park and H. J. Park. “Preparation and characterization of drug-loaded polymethacrylate microspheres by an emulsion solvent evaporation method.†J. Microencapsulation, vol 19, pp. 811–22, 2000.

H. Heiskanen, P. Denifl, P. Pitkänen and M. Hurme. “Effect of concentration and temperature on the properties of microspheres prepared using an emulsion-solvent extraction process.†Adv. Powder Technology, 23(6), 779–786,2011.

H. Tan, J. Wu, D. Huang and C. Gao. “ The design of biodegradable microcarriers for induces cell aggregation.†Macromol Biosci, vol. 10, pp. 156–63, 2010.

M. A Arifin, M. Mel, N. Samsudin, Y. Z. H-Y. Hashim, H. M. Salleh, I. Sopyan and N. Nordin. “Ultra violet/ozone treated polystyrene microcarriers for animal cell culture.†J. Chem. Technol. Biotechnol., vol. 91, pp. 2607-2619, 2015.

T. N. Murakami, Y. Fukushima, Y. Hirano, Y. Tokuoka, M. Takahashi and N. Kawashima. “ Surface modification of polystyrene and poly (methylmetochrylate) by active oxygen treatment.†Appl. Surf. vol. 249, pp. 425–432, 2005.

W. Zhou, G. Ma, and , Z.Su. Microspheres for cell culture. In Ma, G., & Su, Z. (Ed.). Microcapsules in Biotechnology. New York: Taylor and Francis. 2013.

M. A. Sabino. “Oxidation of polycaprolactone to induce compatibility with other degradable polyester.†Poly Degrad and Stab, vol. 92, pp. 986–996, 2007.

C. Wu. “Fabrication and characterization of chitosan microcarrier for hepatocyte culture.†J. Appl. Polym. Sci, vol. 88, pp. 2888-2895,2002.

Yuan S, Xiong G, Roguin A, & Choong C. â€Immobilization of gelatin on poly(glycidyl methacrylate)- grafted polycaprolactone substrates to improve cell material interactions.†Biointerphases, vol. 7, pp. 30, 2012.

B. S. Jacobson, and U. S. Ryan. “Growth of endothelial and HeLa cells on a new multipurpose microcarrier that is positive, negative or collagen coated.†Tissue and Cell, vol. 14,pp. 69–83. 1987)

G.E. Healthcare handbook. Microcarrier Cell Culture principle and methods 2005.

D. O. H Teare, N. Emmison C. Ton-That and R. H. Bradley. “Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet-Ozone Modified Polystyrene Surfaces.†Journal of Colloid and Interface Science, vol. 234, pp. 84–89, 2001.




DOI: http://dx.doi.org/10.18517/ijaseit.8.4-2.7060

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