Morphological and Molecular Identification of Ramie Plants (Boehmeria nivea L. Gaud) in Indonesia

Reni Mayerni, Sari Rukmana Okta Sagita Chan, - Gustian


This research has been conducted at the Experimental Farm of Agricultural Faculty of Andalas University and the Laboratory of the Department of Agronomy and Horticulture Agricultural Faculty, IPB University, Bogor. The purpose of this study was to determine the genetic diversity with similarity analysis of the ramie plant collection of Experimental Farm, Agricultural Faculty of Andalas University. This research was conducted employing morphological and molecular characterization. Morphological characterization of the ramie plant is done by observing the character 17 qualitative and 15 quantitative characters ramie plant. While the molecular characterization using RAPD markers (Random Amplified Polymorphic DNA) with ten primers. The results of morphological characters, which serve as distinguishing characteristics of the five clones, is the color of the leaf. The results of the qualitative character of phenotypic variance analysis of variability obtained plants with broad criteria contained in the 4-character observation (the color of the leaf petiole, the leaf color, flower color, and female sex flowers). Similarity analysis based on morphological characters is qualitatively classified into two groups with the degree of similarity coefficient from 0.34 to 0.75. Similarity analysis of qualitative and quantitative combined data grouped into two groups with the degree of similarity coefficient from 0.02 to 0.20. The similarity analysis based on molecular characters is grouped into two groups with a similarity coefficient level of 0.27 to 0.75.


ramie plants; genetic Diversity; characterization; morphology; RAPD.

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Liu, T., SY. Zhu, L. Fu, QM. Tang, Y. Yu, P. Chen, M. Luan, C. Wang, S. Tang. 2015. Development and characterization of 1.827 Expressed Sequence Tag-Derived Simple Sequence Repeat Markers for Ramie (Boehmeria nivea L. Gaud). PLoS ONE 8(4): e60346.

Zaya DN, Molano-Flores B, Feist MA, Koontz JA, Coons J. 2017. Assessing genetic diversity for the USA endemic carnivorous plant Pinguicula ionantha RK Godfrey (Lentibulariaceae). Conservation Genetics 18(1):171–180 DOI 10.1007/s10592-016-0891-9.

Yu, CM., JR. Chen and YZ. Wang. 2007. Research Progresses of Molecular Breeding in Ramie and Forage Ramie. Plant Fiber Sciences in China 29 (1) : 389–392.

Guo, T., W. She and CX. Xiao. 2012. Research Progress of Ramie for Forage. Crop Research. 6: 730–73.

Liu, T., SY. Zhu, QM. Tang, P. Chen, Y. Yu and S. Tang. 2013. De Novo Assembly and Characterization of Transcriptome Using Illumina Paired-end Sequencing and Identification of CesA Gene in Ramie (Boehmeria nivea L. Gaud). BMC Genomics 14: 125.

Luan, MB., BF. Chen, ZZ. Zou, JJ. Zhu, XF. Wang, Y. Xu, ZM. Sunand and JH. Chen. 2015. Molecular Identity of Ramie Germplasms Using Simple Sequence Repeat Markers. Genetics and Molecular Research 14 (1): 2302-2311.

Liu, L-J., Z-Q. Meng, B. Wang, X-X. Wang, J-Y. Yang and D-X. Peng. 2009. Genetic Diversity among Wild Resources of The Genus Bohmeria Jacq. from West China Determined Using Inter-simple Sequence Repeat and Rapid Amplification of Polymorphic DNA Markers. Plant Production Science 12 (1): 88-96

Fagbemigun, TK., OD. Fagbemi, F. Buhari, E. Mgbachiuzo and CC. Igwe. 2016. Fiber Characteristics and Strength Properties of Nigerian Pineapple Leaf (Ananas cosmosus), Banana Peduncle and Banana Leaf (Musa sapientum) Potential Green Resources for Pulp and Paper Production. Journal of Scientific Research and Reports. 12(2): 1-13.

Kumar, SK., Chakrabarti, S. De and R. Paral. 2016. Eco-Friendly Degumming Technology for Ramie Fiber. Journal of Natural Fiber 13: 227-237.

Kalita, BB., N. Gogoi and MS. Kalita. 2013. Properties of Ramie and Its Blends. International Journal of Engineering Research and General Science 1(2): 1-6

Mayerni, R. 2006. Prospek dan Peluang Tanaman Rami di Indonesia. Andalas University Press. Padang.

Trisiana, LS., T. Maideliza and R. Mayerni. 2016. Kualitas Serat Lima Klon Tanaman Rami. Eksakta 1 (17): 8-16.

Maideliza, T., R. Mayerni and D. Rezki. 2017. Comparative Study Of Length And Growth Rate of Ramie. International Journal on Advance Science Engineering Information Technology. 7(6): 2273-2278.

Yang, S., S. Xue1, W. Kang , Z. Qian Z. Yi. 2019. Genetic diversity and population structure of Miscanthus lutarioriparius, an endemic plant of China. PLOS ONE

Langga, IF., M. Restu and T. Kuswinanti. 2012. Optimalisasi Suhu dan Lama Inkubasi Dalam Ekstraksi DNA Tanaman Bitti (Vitex cofassus Reinw) Serta Analisis Keragaman Genetik Dengan Teknik RAPD-PCR. Jurnal Sains dan Teknologi 12 (3): 265-276.

Govindra, M., M. Vetriventhan and M. Srinivasan. 2015. Importance of Genetic Diversity Assesment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perpectives. Genetic Research International Article ID 431487.

Mayerni, R., Yusniwati, D. Yulfa and SROS. Chan. 2019. Genetic diversity Based on RAPD Marker of Ramie Plants (Boehmeria nivea [L.] Gaud) In West Sumatera. IOP Conf. Series: Earth and Environmental Science 327. IOP Publishing.

Tuteja, N. 2012. Improving Crop Productivity in Sustainable Agriculture. Wiley VCH.

Cholastova, T. and D. Knotova. 2012. Using Morphological and Microsatelite (SSR) Markers to Assess the Genetic Diversity in Alfalfa (Medicago Sativa L.) Agricultural and Biosystems Engineering 6(9): 781-787.

Trivedi, KM., A. Branton, D. Trivedi, G. Nayak, M. Gangwar and S. Jana. 2015. Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd. American Journal of Agriculture and Forestry 3(6): 264-270.

Cortés, SF., ML. Badenes, S. Paz, A. Íñiguez and G. Llácer. 2001. Molecular Characterization of Olive Cultivars Using RAPD Markers. Journal of the American Society for Horticultural Science 126(1): 7–12.

Guasmi, F., W. Elfalleh, H. Hannachi, K. Feres, L. Touil, N. Marzougui, T. Triki and Ferchichi. 2012. The Use of ISSR and RAPD Markers for Genetic Diversity among South Tunisian Barley. ISRN Agronomy :1-10.

Costa, RG., Pereira, I. Garrido, MMT. Sousa and F. Espinosa. 2016. Comparison of RAPD, ISSR, and AFLP Molecular Markers to Reveal and Classify Orchard grass (Dactylis glomerata L.) Germplasm Variations. PlosOne 11(4): e0152972.

Waheed, HM., S. Ali, MS. Khan, MS. Rehman and G. Abbas. 2015. Molecular Genetic Variations among Some Punjab Sheep Breeds Using RAPD Analysis. Advances in Zoology and Botany 3(1): 1-5.

Das, SS., S. Das and P. Ghosh. 2013. Optimization of DNA Isolation and RAPD-PCR Protocol of Acanthus volubilis wall: a Rare Mangrove Plant From Indian Sundarban, for Conservation Concern. European Journal of Experimental Biology 3(6): 33-38

Li, C., S. Chiou, T. Tong, C. Lee, L. Lee and C. Cheng. 2010. Development and Validation of Molecular Markers for Characterization of Bohmeria nivea var. nivea and Bohmeria nivea var. tenacissima. Chinese Medicine 5 (40): 1-9

Conner, J. K. and Hartl, D. L. (2004) A Primer of Ecological Genetics. Massachusetts: Sinauer

Rohlf, F.J. (1998) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System Version 2.0. Applied Biostatistics Inc., Exeter Software, Setauket, New York.

Syukur, M. , Sujiprihati and R. Yunianti. 2012. Teknik Pemuliaan Tanaman. Penebar Swadaya. Jakarta.

Lakitan, B. 2004. Dasar-Dasar Fisiologi Tumbuhan. Rajawali Press. Jakarta



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