Isolates of Lipolytic, Proteolytic and Cellulolytic Bacteria from Palm Oil Mill Effluent and Their Potency as Consortium

Muhammad Said, Muhammad Faizal, Bambang Yudono, - Hasanudin, Sriwi Pertiwi Estuningsih

Abstract


Study of lipolytic, proteolytic and cellulolytic bacteria in variety, especially the study of isolation technique to identify, cultivation technique to produce many researchers have done enzyme and activity test.  While the study on lipolytic, proteolytic and cellulolytic that support the consortium bacteria having the capability of degrading fats, protein and cellulose being the wastewater components have not been conducted yet. This is a novelty of this study.  This study aims to obtain consortium of bacteria having lipolytic, proteolytic and cellulolytic capability from the palm oil mill effluent. The lipolytic, proteolytic and cellulolytic bacteria were isolated from the wastewater using medium mineral (MM), and each of the bacteria was enriched by vegetable oil, casein and Carboxy Methyl Cellulose (CMC). Each of the isolates of bacteria that had been selected was conducted oxygen requirement and synergism tests, and then the consortium of bacteria was wade. Two types of consortium of the bacteria were obtained, that is a facultative anaerobic consortium that consists of two bacteria Bacillus toyonensis strain BCT – 7112 and Stenotrophomonas rhizophila strain e-p10, and the aerobic consortium that consists of three bacteria, namely: Bacillus cereus ATCC 14579, Pseudomonas azotoformans strain NBRC 12693 and Burkholderia cepacia ATCC 25416.

Keywords


bacteria; lipolytic; proteolytic; cellulolytic; consortium.

Full Text:

PDF

References


Ali, N. Hasnain, W. Amna, Gul-e-Sahra and A. Tauseef, Screening of oil contaminated soil for isolation of lipids degrading bacteria, Sci.Int. 26(4) (2014) 1595-1600.

J.L. Arpigny and J. Karl-Erich, Bacterial lipolytic enzymes: classification and characteristics. Biochem. J. 343(1999)177-183,

N.C. Bestari and Suharjono, Activity Assay and Identification of Lipolytic Bacteria krom Wastewater Fish Industry at District Muncar, Banyuwangi Indonesia, Int.J. ChemTech Res. 8(11)(2015)377-383.

A.L Giudice, L. Michaud, D. de Pascale, M. De Domenico, G. di Prisco, R. Fani and V. Bruni1, Lipolytic activity of Antarctic cold-adapted marine bacteria (Terra Nova Bay, Ross Sea), Journal of Applied Microbiology 101(2006)1039–1048.

L.P. Lee, M.K. Hudzaifah, C. Marimuthu, Subash C. B. Gopinath, L. Thangavel and T. Thean-Hock, Lipase-Secreting Bacillus Species in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution, BioMed Research International (2015)1-9.

C.K. Lodha, S. Kumar and R. S. Awasthi, Screening of Lipolytic Fungi from Oily Premises for Biosurfactant Production, World Journal of Pharmacy and Pharmaceutical Sciences 5(5)(2016)1176-1181.

B. Mandepudi, V.C. Ghanta and D. Mandepudi, Fermentation of vegetable oils in the light of lipolytic activity with lipase producing bacterial isolates from soil samples, International Journal of Pharmaceutical Applications 3(2)(2012)352-359.

A.T. Odeyemi, B.I. Aderiye and O.S. Bamidele, Lipolytic activity of some strains of klebsiella, pseudomonas and staphylococcus spp. from restaurant wastewater and receiving stream, Journal of Microbiology Research 3(1)(2013)43-52.

P.Pallavi, T. Ravikumar and S. Ram Reddy, Isolation and characterization of lipase producing bacteria for biodegradation of oil contamination, Int. J. Curr. Res. Aca., Rev 3(8)(2015) 56-66.

N.T. Phong, N.T. Duyen, C.N. Diep, Isolation and characterization of lipid-degrading bacteria in wastewater of food processing plants and restaurants in can tho city, Vietnam, American Journal of Life Sciences 2(6)(2014)382-388.

O.N. Donkor, A. Henriksson, T. Vasiljevic, N. P. Shah, Proteolytic activity of dairy lactic acid bacteria and probiotics as determinant of growth Ana in vitro angiotensin-converting enzyme inhibitory activity in fermented milk, Le Lait 87(1)(2007)21-38.

M.P. Rodarte, D. R. Dias, D.M. Vilela and R.F. Schwan, Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L.) Maringá, 33(3)(2011)457-464.

S.V. Megha and B. Annadurai. Isolation and Identification of Proteolytic Bacteria From Raw Milk Samples, G.J.B.B, 3(4)(2014) 391-397.

D. Rupali, Screening and Isolation of Protease Producing Bacteria from Soil Collected from Different Areas of Burhanpur Region (MP) India, Int.J.Curr.Microbiol.App.Sci. 4(8)(2015)597-606.

A. Balamurugan, R. Jayanthi, P. Nepolean, R.V. Pallavi and R. Premkumar, Studies on cellulose degrading bacteria in tea garden soils, African Journal of Plant Science 5(1)(2011)22-27.

S.K. Dubey, R. K. Meena, S. Sao, J. Patel, S. Thakur and P. Shukla, Isolation and haracterization of Cellulose Degrading Bacteria from Biogas Slurry and their RAPD profiling, Curr Res Microbiol Biotechnol. 2(4)(2014)416-421.

S. Hatami, H.A. Alikhani, H. Besharati, N. Salehrastin, M. Afrousheh and Z.Y. Jahromi, Investigation on Aerobic Cellulolytic Bacteria in Some of North Forest and Farming Soils, American-Eurasian J. Agric. & Environ. Sci. 3(5)(2008)713-716.

S. Huang, P. Sheng and H. Zhang, Isolation and Identification of Cellulolytic Bacteria from the Gut of Holotrichia parallela Larvae (Coleoptera: Scarabaeidae), Int. J. Mol. Sci. 13(2013)2563-2577.

M. Irfan, A. Safdar, Q. Syed and M. Nadee, Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity, Turk J Biochem. 37(3)(2012)287–293.

M. Wenzel, I. SchoÈ nig, M. Berchtold, P. KaÈmpfer and H. KoÈ nig, Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis, Journal of Applied Microbiology. 92(2002)32-40.

S. D. Todorov, S. Stojanovski, I. Iliev, P. Moncheva, L. A. Nero and I. V. Ivanova, Technology and safety assessment for lactic acid bacteria isolated from traditional Bulgarian fermented meat product “lukanka”, Brazilian Journal of Microbiology 48(2017)576-586.

Y. Ahmed, Z. Yaakob, P. Akhtar and K. Sopian, Production of biogas and performance evaluation of existing treatment processes in palm oil mill effluent (POME), Renewable and Sustainable Energy Reviews 42(2015)1260-1278.




DOI: http://dx.doi.org/10.18517/ijaseit.9.2.4938

Refbacks

  • There are currently no refbacks.



Published by INSIGHT - Indonesian Society for Knowledge and Human Development