Membrane Performance of Micro and Ultrafiltration on Folic Acid Separation from Dent Corn (Zea mays var. indentata) Hydrolyzed by Rhizopus oligosporus-C1

- Aspiyanto, Agustine Susilowati, Puspa Dewi Lotulung, Yati Maryati


Performance of microfiltration (MF) and ultrafiltration (UF) membranes to separate of folic acid from corn (Zea mays var. indentata) hydrolysate equipped in stirred filtration cell (SFC) mode were performed as a reference guide toward semi pilot scale. Separation on hydrolysate suspension of yellow dent corn (HSYCD) and hydrolysate suspension of white dent corn (HSWCD) as a results of hydrolysis of protease enzyme of Rhizopus oligosporus strain-C1 0.025 % and 0.075 % (w/w, dissolved protein) at pH 5 and 30 °C for 24 hours was conducted by MF (0.45 µm) and UF (100000 MWCO) membranes at stirrer rotation speed 400 rpm and trans-membrane pressure (TMP) of 20, 30 and 40 psia for 30 minutes. The experimental result showed that based on optimization of fluxes, the best performances of MF and UF membranes on HSYDC and HSWDC were achieved at TMP 40 psia and gave fluxes of 0.0534 and 0.0508 mL/cm2.min., respectively. In these process conditions, it take place an increase of folic acid in concentrates of HSYDC and HSWDC compared with before process (feed). Identification of molecular weight (MW) on folic acid from HSYDC and HSWDC displayed dominant folic acid monomer at T2.7, and T2.4 and T2.89. This matter showed that commodity of yellow dent corn has more potential use as source of folic acid compared with white dent corn at both similar condition of hydrolysis and separation and/or purification processes.


membrane; folic acid; dent corn (Zea mays var. indentata); retentate; permeate.

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