Influence of Different Concentration of Tris Buffer Solution on Calcium Carbonate Precipitation in Bio-based Repair Materials

Prima Yane Putri, Keiyu Kawaai, Isao Ujike, Hiroshi Okuno


This research examined differences in the concentration of Tris buffer solution on calcium carbonate precipitation in bio-based repair materials. Four concentrations of Tris buffer solution were chosen based on previous research with initial pH 8.0 and 9.0. Initial experiments demonstrated that the concentration and pH of Tris buffer solution had an influence on the amount of precipitation of calcium carbonate. X-Ray Diffraction (XRD) analysis elucidated the morphological and structural differences of the calcium carbonate crystal, including calcite and vaterite. They are the prominent forms of CaCO3 detected based on results obtained by according to FT-IR analysis. The result further explains the effectiveness of Tris buffer concentration.


Bio-based repair materials; tris buffer solution; calcium carbonate precipitation; fourier-transformed infra-red spectroscopy (FT-IR); X-Ray riffraction (XRD).

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Published by INSIGHT - Indonesian Society for Knowledge and Human Development