Alginate Encapsulation of Trichoderma harzianum as Biocontrol Agent against Brown spot Disease on Rice (Oryza sativa) in Vitro Assays

Intan Sakinah Mohd Anuar, Ku Asmah Ku sulong, Husein Abdul Gani, Nur Nadia Safi’n, Khairul Azman Samsudin, Mohd Zafri Wahab


Brown spot caused by Bipolaris oryzae is an important rice disease which can cause a reduction in yield and grain quality in Malaysia. The application of chemical agents in controlling the brown spot disease can cause undesirable effects such as residual toxicity, environmental pollution, and development of pathogen resistance to fungicides. Instead, biocontrol methods have to be developed to ensure environmental safety, the longevity of usage while effective methods of plant disease management. However, to the best of our knowledge, no research has been conducted about alginate encapsulation of Trichoderma harzianum. Therefore, the objectives for this study is to measure the performance of encapsulation of Trichoderma harzianum isolates in controlling the brown spot disease on rice. Alginate encapsulation was produced from conidial suspension by adding sodium alginate and calcium chloride which result in small beads. The isolate was isolated and characterized based on morphological characters and have been proved by pathogenicity tests. Based on the results dual culture test showed the highest percentage inhibition of radial growth which was 85.07% with 10 replication of Bipolaris oryzae. The findings from this study can be used as an alternative method to control brown spot disease which will render easier application to rice plantation in the future which suit for commercial purposes and contamination-free environment.


Bipolaris oryzae; Trichoderma harzianum; alginate encapsulation.

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M. Kubo, & M. Perevdori, “The Future of Rice Production and Consumptionâ€. Journal of Food Distribution Research, 35(1), 129-142, 2004

H. Azimi, B. Abu Samah, & N. Tiraieyari. “Organic Farming and Sustainable Agriculture in Malaysia: Organic Farmers' Challenges toward Adoptionâ€. Asian Social Science, 10(4). doi:10.5539/ass.v10n4p1, 2014

A. Sivanesan (1987). “Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs.†Mycological Papers. 158:1-261, 1987

D. O. Agriculture. Paddy production survey report Malaysia: Main Season 2009. Department of Agriculture Peninsular, Putrajaya, Malaysia, 2009.

Y. Zhu, Y. Wang, H. Chen,, & B. Lu. Conserving Traditional Rice Varieties through Management for Crop Diversity. BioScience Journal, 53(2), 158-162, 2003 doi: 10.1641/0006-3568(2003)

M.A. Marchetti and H.D. Peterson, 1984. “The role of Bipolaris oryzae in floral abortion and kernel discoloration in riceâ€. Plant Dis., 68: 288–91, 1984

H.H. Chan, D. Sivaram, C.W. Chung, K. Asrore, and N.K. Ho. “Direct Seeding-Impact and Implications on Rice Cultivation Practices in Peninsular Malaysia. Paper presented in Workshop on Water and Direct Seeding for Riceâ€, June 1993. Jabatan Pengairan dan Saliran Malaysia (JPS).

M. D. Asante, “Grain Quality and Determinants of Farmer's Preference for Yield Varietal traits in Three Districts of Ghana: Implications For Research and Policy.†Journal of Development and Agricultural Economics, 5(7), 284-294, 2013.

Anon. Padi Production Survey Reports Malaysia. Main Season 1992/ 1993 and Off Season 1993. Jabatan Pertanian, Semananjung, Malaysia, 1993.

G. Faruq, M. Hadjim, M,C. A. Meisner. Kernel Ageing: An Analysis in Four Malaysian Rice Varieties. International Journal of Agriculture & Biology 1560–8530/2003/05–3–230–232, 2003

M. R. Safari-Motlagh, and B. Kaviani. “Characterization of new Bipolaris spp.: The causal agent of rice brown spot disease in the north of Iranâ€. International Journal of Agriculture and Biology 10:638-642, 2008

L. J. Dallagnol, F.A. Rodrigues, M. V. B. Mielli, J. F. Ma, Datnoff, L. E. “Defective active silicon uptake affect some components of rice resistance to brown spot.†Phytopathology, 99:116-121., 2009

I.H. Mian, and Fakir, G.A. Fakir, “Fungi moisture content and germ inability of rough rice seeds during storage.†Seed Research, 17(2): 169-173, 1989

M.S. Ali, and B. Deka, “Role of fungi causing grain discoloration of rice and management under storageâ€..Indian Journal of Mycology and Plant Pathology. 26(1):79-85, 1996

M.M. Kamal and M.A.T. Mian. “Diversity and pathogenicity of the rice brown spot pathogen in Bangladesh assessed by genetic fingerprint analysis.†Bangladesh J.Bot. 38(2):119-125, 2009.

Anonymous. Standard Evaluation System for rice. Pp.54. International Rice Research Institute,Manila, Philippines.1, 1996.

A. Panneerselvam and S. Selvarajkalaiselvi. Int. J. Appl. Bio.and Pharmaceutical tec.2(1)179-183, 2011

S. Gomathinavagam, S.A. Persaund, M. Rekha. “Comparative study of biological agents, Trichoderma harzianum and Trichoderma viridae for controlling brown spot disease in rice.†Jbiopest: 28-32, 2012.

G.M. Abdel-Fataah, Y.M. Shabana, A.E. Ismail, Y.M. Rashad. “Trichoderma harzianum: a biocontrol agent against Bipolaris oryzae.†Mycopathol, 164, 81-99, 2007.

R. Baker, “Trichoderma spp. as plant-growth stimulantsâ€. Biotechnol. 7(2), 97–106. [ Links ]Benitez, T.; Rincon, A.M.; Limon, M.C.; Codon, A.C. Biocontrol mechanisms of Trichoderma strains. Int. Microbiol. 7(4), 249–260, 2004.

A. Pannerpersalam and Saravanamuthu, R. Saravanamuthu. Ind.J.Geobios., 26:126-26, 1999

G.A Harman, & D.B. Custis, “Formulation of viable microorganism and their methods of production and use.†Grant US9090884 B2, 2015

M.B. Ellies, Commonwealth Mycological Institute; Kew, UK: Dematiaceous Hyphomycetes, 1971

IRRI, (2015). Standard evaluation system for rice. International Rice Institute, Los Banos, Manila, Philippines

A. Zibaee. Rice: Importance and Future. J Rice Res 1:e102, 2013S. Harish, D. Saravavakumar, R. Radiacommar, E.G., Ebenezar, K. Seetharaman, “Use of plant extracts and biocontrol agents for the management of brown spot disease in riceâ€. Biocontrol 53(3), 555-567, 2007

S. Kumari. “Morphological characterization of Bipolaris oryzae causing brown spot of paddy in Biharâ€, 85-87, 2015

B. Ratika & U. Utpal. “An overview of fungal and bacterial biopesticides to control plant pathogens/diseasesâ€. African Journal of Microbiology Research, 8(17), 1749-1762. 2014

P. Saranraj, P. Sivasakthivelan, S. Sivasakthi. “Prevalence and production of plant growth promoting substance by Pseudomonas fluorescens isolated from paddy rhizosphere soil of Cuddalore district, Tamil Nadu, India.†African J. of Basic and App. Sciences, (5) 95-101, 2013

S. Nazari., M. Yarran-Nikkhah, K. B. Fotouhifar, V. Khosrari, A. Alizadeh. “Bipolaris spesies associated with rice plant: Pathogenicity and genetic diversity of B. oryzae using rep-PCR in Mazauda Province of Iran.â€, 4(4):497-508, 2015.

G.E. Harman. “Overview of mechanisms and uses of Trichoderma spp.†Phytopathology 96, 190-194, 2006

M. Angelica, G. Barbosa, K. G. Rehn, M. Menezes, R.R. Mariano. “Antagonism of Trichoderma sp. on Cladosporium Herbarum and their enzymatic characterizationâ€. Braz J Microbiol; 32:98–104, 2001

V. Manimegalai, V. Ambikapathy, A. Panneerselvam. “Biological control of paddy brown spot caused by Bipolaris oryzae (Breda de Haan).†1 (4): 24-28. 2011.

M. Vincekovic, M. Enjak, N. J., S. Topolovec-Pintaric, E. Äermic, E., Bujan, M., Juric, S. “Encapsulation of Biological and Chemical Agents for Plant Nutrition and Protection: Chitosan/Alginate Microcapsules Loaded with Copper Cations and Trichoderma virideâ€. J. Agric. Food Chem. 2016, 64, 8073−8083, 2016



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