Effect of Fermentation Time and Calcium Nitrate Concentration on Enzyme Glucoamylase Production of Gliocladium KE Using Sago Hampas Solid Substrate

Abstract

This research aims to obtain the appropriate fermentation time and suitable concentration of calcium nitrate (Ca (NO3) 2) which is suitable with minerals solution used for sago hampas as a solid substrate to produce optimal activity of glucoamylase. This study was conducted in two stages. The first stage is to apprehend the fermentation time of Gliocladium KE fungal on solid substrates to produce optimal glucoamylase enzyme activity. Fermentation was conducted in 6 days, which each day is measured enzyme activity of glucoamylse and counted the number of spores of Gliocladium KE. The second stage is to get the optimal concentration of calcium nitrate was added to the mineral solution on a solid substrate of sago hampas to produce the highest glucoamylase. The concentration of calcium nitrat used was 2.57 g, 3.57 g and 4..57 g. The results showed that the fermentation for 4 days produced the highest glucoamylase enzyme activity of 24.22 units / mL by the number of spores 2.7 x 10 8 spores / mL. The concentration of calcium nitrat on 3:57 g with fermentation for 4 days resulted in the highest enzyme glucoamylase.