Exploration and Identification of the Indigenous Arbuscular Mycorrhizae Fungi (AMF) in the Rhizosphere of Citronella (Andropogon nardus L.) in the Dry Land Regions in West Sumatra Province, Indonesia

- Armansyah, Aswaldi Anwar, Auzar Syarif, - Yusniwati, Rudi Febriamansyah


Climate change has an impact on the transition function of the wetland to dry land. This shift led to the increased of the dry land area with less potential for food crops and horticulture. Citronella plants have been identified as one of the lemongrass crops that able to survive in the dry land area. However, the resulting yield to produce citronella oil is still very low at 0.8 to 1.0%. In this regard, Arbuscular Mycorrhizae Fungi (AMF) was indicated to be able to increase the absorption of nutrients and water in many plants in the dry land regions.  The type of AMF that compatible enough with citronella plant was not identified yet. The purpose of this study was to determine the number and diversity of AMF in the rhizosphere of indigenous citronella (lemongrass). The study was conducted from August to November 2015 in three regions of cultivation of citronella using survey methods and wet screening. The study found four important AMF geniuses in the study site; there are Glomus, Acaulospora Gigaspora, and Sclerocystis.


climate change; indigenous mycorrhizae; rhizosphere; citronella plants; dry land

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R. Febriamansyah, Refdinal, Yusmarni, and L. Hanum. “The climate change and the lost of the primary economic sources of rain-fed paddy farmers: a case study from Nagari Simawang, West Sumatera Indonesia”. In Proceeding of the International Workshop on Agribusiness: Entrepreneurship and Innovation for Food Security and Rural Development. Bogor, 5-6 December 2012 in Bogor, Indonesia, 2012.

Daswir dan I. Kusuma, “Citronella (Andropogon nardus L.) development of the Sawah Lunto West Sumatra”. Bulletin research spice plants and medicine. 2006. Vol. XVIII No.1.12-22.

J.T .Yuhono.and S. Suhirman. ” Increased rendemen strategy and quality of Pogostemon cablin Benth in agribusiness patchouli”. research centers medicinal plants and aromatic. litbang balittro. 2006.

Prianto, R Ritnowati, and Juswono. “Isolation and characterization from oil clove flowers ( Syzygium aromatic ) dried the results of distillation the vapor”. Student Journal, Vol. 1, No. 2, pp. 269-275. The university of Brawijaya Malang. 2013.

S.Munawaroh and P.A. Handayani. “The extraction of oil leaves Citrus hysteria With a solvent and n-heksana ethanol”. Journal competence technique Vol. 2, No.1, 2010.

B.H. Prasetyo dan D.A. Suriadikarta. Characteristic, “The potential and technology land management ultisol to the development of agriculture dry land in Indonesia” Journal agricultural research, 25(2). 2006.

N. Hakim. The management of soil fertility sour with technology granting lime integrated, Andalas University Press. 2006.

R. Boer. Insurance climate protection security guarantees farmers to climate change. Centre for Climate Risk and Opportunity Management in Southeast Asia and Pacific Bogor Agricultural University (CROM SEAP IPB). 2012

B.Irawan. “Anomalous phenomenon el Nino weather event and la Nina: long-term trends and its effect on food production “. Forum Penelitian Agro Ekonomi. Volume 24 No 1. 2006.

Sutomo, S. “Data analysis conversion and by prediction needs land .pages 135-149 in the results round table 2 control conversion and development agricultural land. The directorate of the expansion of acreage”. The production of food crops.Department of agriculture. Jakarta. 2004.

Zainal, Daswir, I. Kusuma, M. Ramadhan, dan D. Allorerung. “Agribusiness development Citronella (Andropogon nardus L.) insightful conservation in the city Sawah Lunto, West Sumatra. A final report “. Cooperation central research and development of the estate and of city government Sawahlunto. pp. 24-26. 2004.

Daswir. “The role of Citronella (Andropogon nardus L.) as a plant conservation on of plantations cocoa in critical areas ”. Research center medicinal plants and aromatic”. Newsletter. Littro. Vol. 21 No 2. 2010.

J..W. Kloepper. “Plant growth promoting rhizobacteria as biological control agents “. pp. 255-274. In F.B. Meeting, Jr. (Ed.). Soil Microbial Ecology, Applications in Agricultural and Environmental Management. Marcel Dekker, Inc. New York. 1993..

Y. Rouphael, P. Franken, C. Schneider, D. Schwarz, M. Giovannetti, M. Agnolucci, S. D. Pascale, and P. Bonini, “Arbuscular mycorrhizal fungi act as biostimulants in horticultural crops,” Scientia Horticulturae. Volume 196, Pages 91–108. Biostimulants in Horticulture 2015.

P. du Jardin, “Plant biostimulants: Definition, concept, main categories and regulation”, Scientia Horticulturae. Volume 196, Pages 3–14. Biostimulants in Horticulture 2015.

M. Halpern, A. Bartal, M. Ofek, D. Mintz, T. Muller, and U. Yermiyahu,”The use of biostimulants for enhancing nutrient uptake”, D.L. Sparks (Ed.), Advances in Agronomy, Vol. 129 (2015), pp. 141–174, 2015.

L. Kohl, E. Catherine. Lukasiewicz, and M. G. A. V. D. Heijden, “Establishment and effectiveness of inoculated arbuscular mycorrhizal fungi in agricultural soils,” Plant, Cell & Environment. Volume 39, Issue 1 January 2016, 136–146, 2016.

A. Saidi, E. F. Husin , A. Rasyidin., Eddiwal and L Ismon. Effect of Arbuscular Mycorrhiza Fungi (AMF) and The Organic Material to The Glomalin Production and The Soil Physical Properties of Ultisols. International Journal of Advanced Science, Engineering and Information Technology (IJASEIT).Vol.5. No. 4. (2015)

S. E. Smith and D.J. Read. Mycorrhizal Symbiosis, New York: Academic Press. 1997.

B. Mosse, Vesicular-arbuscular mycorrhizae research for tropical agriculture, Hawaii Institute of tropical agriculture and human resources. England. 77 pp, 1981.

(2016) Google Earth, https://www.google.com/earth/ Accessed on 9 July 2016.

M. Brundrett, N. Bougher, B. Dell, T. Grove, and N. Malajczuk. Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research (ACIAR). Canberra, Australia. 1996.

(2003), Invam, Collection culture of arbuscular and vesicular mycorrhizal fungi (Available online at http://invam.caf.wvu.edu/mycinfo/taxonomy/classification.htm: 18 Aug 2003).

P. Kormanik, and A. C. McGraw, Quantification of VesikularArbuskular Mycorrhizae in Plant Roots. Dalam Schenck, N. C. (Eds.) Methods and Principles of Mycorrhizal Research. The American Phytopathological Society. 1982.

A. Trouvelot, J.L. Kough, and V. G. Pearson, Mesure du Taux de mycorrhization VA due système radicular. Recherche de méthodes destination agent one signification function Nelle, In Physiological and genetical aspects of mycorrhizae. Gianinazzi-Pearson V. et Gianinazzi S. (Eds.), INRA edition, Paris,1986.

Giovannetti, M. Avio, L. Sbrana C, and AS. Citemesi. Factors affecting appressorium development in the vesicular arbuscular mycorrhizal fungus Glomus mossie (Nicol. & Gerd.) Gerd. & Trappe. New Phytol., 123, 115~122. 1993.

A. Pinior, U.Wyss, Y. Piché, and H. Vierheilig. Plants colonized by AM fungi regulate further root colonization by AM fungi through altered root exudation. Can. J. Bot. 77: 891–897.1999.

L.Herrmann, D. Lesueur, L. Bräu, J.Davison, T. Jairus, H. Robain, A. Robin, M. Vasar, W. M Öpik. The diversity of root-associated arbuscular mycorrhizal fungal communities in a rubber tree plantation chronosequence in Northeast Thailand. Jurnal Mycorrhiza Volume 26, Issue 8, pp 863–877. 2016.

Setiadi Y. 2000. The status of research and the use of boletus mycorrhizal arbuscular and rhizobium land relegated to rehabilitate. Preceding national seminar mycorrhizal I. Bogor 15 – 16 November 1999.

G. Leon,M Moora,L Neuenkamp, M.Vasar,C.G.Buerno,J.Davison and M.Zobel, Symbiont dynamics during ecosystem succession: co-occurring plant and arbuscular mycorrhizal fungal communities. Microbiology Ecology, 2016, Vol. 92, No. 7. 2016.

R.B. Clark, Arbuscular mycorrhizal adaptation, spore germination, root colonization, and host plant growth and mineral acquisition at low pH. Plant Soil 192:15-22. 1997.

AH Fitter, A Heinemeyer, R Husband, E Olsen, K P Ridgway, and P L Staddon. Global environmental change and the biology of arbuscular mycorrhizas: gaps and challenges. Canadian Journal of Botany, Vol. 82, No. 8: pp. 1133-1139. 2004,

E. K. Holste1, K. D. Holl, R. A. Zahawi, and R. K. Kobe1. Reduced aboveground tree growth associated with higher arbuscular mycorrhizal fungal diversity in tropical forest restoration. Ecology and Evolution 6: 7253–7262. 2016.

F. He, M. Tang, S. L. Zhong, R. Yang, L. Huang, and H. Q. Zhang. Effects of soil and climatic factors on arbuscular mycorrhizal fungi in rhizosphere soil underRobinia pseudo-acacia in the Loess Plateau, China. Jurnal soil science. Volume 67, Issue 6 November 2016 Pages 847–856. 2016.

A. Saidi, E.F. Husin, A. Rasyidin, Eddiwal and L Ismon, Selection of Arbuscular Mycorrhizal Fungi (AMF) Indigenous in Ultisol for Promoting The Production of Glomalin and Aggregate Formation Processes. International Journal of Advanced Science, Engineering and Information Technology (IJASEIT). Vol.4. No. 6. 2014.

E. Verbruggen, M. G. A. Heijden, M. C. Rillig, and E. T. Kiers. The mycorrhizal fungal establishment in agricultural soils: factors determining inoculation success. New Phytologist 197. 1104–1109. 2013.

M Opik, and M Moora. Missing nodes and links in mycorrhizal networks. New Phytologist 194: 304–306. 2012.

D. TrejoI. Barois, and W. S. Conde. Disturbance and land use effect on the functional diversity of the arbuscular mycorrhizal fungi. Journal Agroforestry Systems, Volume 90, Issue 2, pp 265–279. 2016.

DOI: http://dx.doi.org/10.18517/ijaseit.8.1.2363


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