Effect of Various Cultivation Methods on Cellobiohydrolase Production from Aspergillus niger

Abstract

Three different cultivation methods, i.e., shaking-flask culture (SFC), static surface liquid culture (SSLC) and membrane surface liquid culture (MSLC) were used to cultivate the filamentous fungi,  Aspergillus niger PY11 in order to differentiate its behaviours by different cultivation methods using the same media, by determination of growth profile during fermentation. The dry cell weight, protein concentration, cellobiohydrolase (CBH) activity and residual sugar concentration in SSLC and MSLC were 1.5 to 2-folds than that by SFC. Cultivation of A. niger using MSLC was higher than SSLC with maximum biomass concentration, protein concentration and enzyme activity were 0.93 g dry weight/ml medium, 3.49 mg/ml and 36.99 U/ml respectively. MSLC possess the best growth characteristics and was the best cultivation method in production of CBH from A. niger PY11.