The Use of Tragacanth as a Gelling Agent in Roselle Flower Extract based Radiochromic Indicators

Goib Wiranto, Slamet Widodo, I Dewa Putu Hermida, Yudi Y. Maulana, Deni P. Kurniadi, Shenny Oktaviana, Atiek R. Noviyanti, - Juliandri

Abstract


Gamma irradiation techniques have been used in various fields, such as sterilizing medical equipment and food products. In such a process, monitoring the dose of radiation using radio radiochromic dosimeter is important to guarantee the accuracy of the radiation dose absorbed by the irradiated materials. Radio chromic dosimeters usually consisted of radiation-sensitive dyes that could change colors when exposed to radiation. In this research, natural dyes in the form of anthocyanins obtained from roselle flower extract (Hibiscus sabdariffa L.) have been used as radio chromic indicators. The radio chromic indicators have been made in liquid using water solvent and gel using a tragacanth gelling agent. Each radiochromic indicator's pH values varied between 2 and 11 using NaOH and HNO3 solutions. All radio chromic indicators were then gamma irradiated using a Co-60 radiation source and tested for stability at 8°C under closed storage conditions. The characterization was performed using UV Vis spectrophotometry. The results showed that roselle flower extract was sensitive to gamma radiation as indicated by decolorization, and their sensitivity increased when made in the form of gel at pH 2 and 5. The stability test also showed that the anthocyanin content was stable after being maintained for 28 days in a closed storage at 8°C. With such differences in sensitivity of the roselle flower extract when used in either liquid or gel solution, the radio chromic indicators should find wide application in the future, depending on the radiation doses given to the solutions.

Keywords


Radio chromic indicator; tragacanth gel; roselle flower extract; gamma radiation; natural dyes

Full Text:

PDF

References


A. T. Naikwadi, B. K. Sharma, K. D. Bhatt, and P. A. Mahanwar, “Gamma Radiation Processed Polymeric Materials for High Performance Applications: A Review,†Frontiers in Chemistry, vol. 10. Frontiers Media S.A., Mar. 14, 2022, doi: 10.3389/fchem.2022.837111.

J. Ji, Z. Allahdad, E. Sarmast, S. Salmieri, and M. Lacroix, "Combined effects of microencapsulated essential oils and irradiation from gamma and X-ray sources on microbiological and physicochemical properties of dry fermented sausages during storage," LWT, vol. 159, Apr. 2022, doi: 10.1016/j.lwt.2022.113180.

R. Indiarto, A. N. Irawan, and E. Subroto, "Meat Irradiation: A Comprehensive Review of Its Impact on Food Quality and Safety," Foods, vol. 12, no. 9, p. 1845, Apr. 2023, doi: 10.3390/foods12091845.

P. Bhatnagar et al., "Impact of irradiation on physico-chemical and nutritional properties of fruits and vegetables: A mini review," Heliyon, vol. 8, no. 10. Elsevier Ltd, Oct. 01, 2022, doi: 10.1016/j.heliyon.2022.e10918.

V. S. Chernonosova et al., "Effect of Sterilization Methods on Electrospun Scaffolds Produced from Blend of Polyurethane with Gelatin," J. Funct. Biomater., vol. 14, no. 2, Feb. 2023, doi: 10.3390/jfb14020070.

H. Yasuda and H. Yoshida, "Application of a Portable Colorimeter for Reading a Radiochromic Film for On-Site Dosimetry," Appl. Sci., vol. 13, no. 8, Apr. 2023, doi: 10.3390/app13084761.

C. Guardiola, A. Márquez, M. C. Jiménez-Ramos, J. G. López, A. Baratto-Roldán, and X. Muñoz-Berbel, "Dosimetry with gafchromic films based on a new micro-opto-electro-mechanical system," Sci. Rep., vol. 11, no. 1, Dec. 2021, doi: 10.1038/s41598-021-89602-9.

P. Casolaro, "Radiochromic films for the two-dimensional dose distribution assessment," Applied Sciences (Switzerland), vol. 11, no. 5. MDPI AG, pp. 1–16, Mar. 01, 2021, doi: 10.3390/app11052132.

A. AL Zahrany, K. Rabaeh, M. Eyadeh, and A. Basfar, "Dosimetric evaluation of methyl red radiochromic film for radiation processing," Pigment Resin Technol., vol. 50, no. 2, pp. 157–162, Mar. 2021, doi: 10.1108/PRT-02-2020-0008.

K. Rabaeh and A. Basfar, "Optical evaluation of dithizone solution as a new radiochromic dosimeter," Pigment Resin Technol., vol. 49, no. 4, pp. 249–253, Jun. 2020, doi: 10.1108/PRT-10-2019-0091.

I. Fitilis et al., "Polymer-Gel Radiation Dosimetry of Laser-Based Relativistic Electron Sources for Biomedical Applications: First Qualitative Results and Experimental Challenges," Front. Phys., vol. 10, Apr. 2022, doi: 10.3389/fphy.2022.727511.

C. C. Bueno et al., "Performance Characterization of Dosimeters Based on Radiation-Hard Silicon Diodes in Gamma Radiation Processing," Front. Sensors, vol. 3, Jan. 2022, doi: 10.3389/fsens.2022.770482.

M. M. Skowyra, C. Ankjærgaard, L. Yu, L. R. Lindvold, A. L. Skov, and A. Miller, "Glass transition temperature of Risø B3 radiochromic film dosimeter and its importance on the post-irradiation heating procedure," Radiat. Phys. Chem., vol. 194, May 2022, doi: 10.1016/j.radphyschem.2022.109982.

Y. S. Soliman, A. A. Abdel-Fattah, and S. G. Dutremez, "1,10-Bis(diphenylamino)-4,6-decadiyne as an active component of a radiochromic film dosimeter," Radiat. Phys. Chem., vol. 189, Dec. 2021, doi: 10.1016/j.radphyschem.2021.109714.

I. Xhaferllari et al., "Clinical utility of Gafchromic film in an MRI-guided linear accelerator," Radiat. Oncol., vol. 16, no. 1, Dec. 2021, doi: 10.1186/s13014-021-01844-z.

K. A. Rabaeh, S. A. Aljammal, M. M. Eyadeh, and K. M. Abumurad, "Methyl thymol blue solution and film dosimeter for high dose measurements," Results Phys., vol. 23, Apr. 2021, doi: 10.1016/j.rinp.2021.103980.

M. Scotti et al., "How Xylenol Orange and Ferrous Ammonium Sulphate Influence the Dosimetric Properties of PVA–GTA Fricke Gel Dosimeters: A Spectrophotometric Study," Gels, vol. 8, no. 4, Apr. 2022, doi: 10.3390/gels8040204.

D. Ariyanti and W. Saputri, "Synthesis of bio-polymer based chitosan and starch with methyl orange dyes as a material potential for low dose gamma film dosimeter," in Journal of Physics: Conference Series, Feb. 2020, vol. 1436, no. 1, doi: 10.1088/1742-6596/1436/1/012018.

N. Ghareaghajlou, S. Hallaj-Nezhadi, and Z. Ghasempour, "Red cabbage anthocyanins: Stability, extraction, biological activities and applications in food systems," Food Chemistry, vol. 365. Elsevier Ltd, Dec. 15, 2021, doi: 10.1016/j.foodchem.2021.130482.

G. M. Tazoho, E. E. Agbor, and I. Gouado, "In vitro, antioxidant activities of aqueous and methanol roselle (Hibiscus sabdariffa) calyces extracts from two localities in Cameroon," North African J. Food Nutr. Res., vol. 4, no. 8, pp. 292–297, Sep. 2020, doi: 10.51745/najfnr.4.8.292-297.

N. H. Indah, A. Listyarini, and C. Imawan, "Natural Red Dyes from Hibiscuss sabdariffa L. Calyxes Extract for Gamma-Rays Detector," in Journal of Physics: Conference Series, Jan. 2020, vol. 1428, no. 1, doi: 10.1088/1742-6596/1428/1/012061.

B. Enaru, G. Drețcanu, T. D. Pop, A. Stǎnilǎ, and Z. Diaconeasa, "Anthocyanins: Factors affecting their stability and degradation," Antioxidants, vol. 10, no. 12. MDPI, Dec. 01, 2021, doi: 10.3390/antiox10121967.

B. Alappat and J. Alappat, "Anthocyanin pigments: Beyond aesthetics," Molecules, vol. 25, no. 23. MDPI AG, Dec. 01, 2020, doi: 10.3390/molecules25235500.

A. Dudek, M. Spiegel, P. Strugała-Danak, and J. Gabrielska, "Analytical and Theoretical Studies of Antioxidant Properties of Chosen Anthocyanins; A Structure-Dependent Relationships," Int. J. Mol. Sci., vol. 23, no. 10, May 2022, doi: 10.3390/ijms23105432.

Z. Huang et al., "Preliminarily exploring of the association between sugars and anthocyanin accumulation in apricot fruit during ripening," Sci. Hortic. (Amsterdam)., vol. 248, pp. 112–117, 2019, doi: 10.1016/j.scienta.2019.01.012.

S. A. Amer et al., "Potential Effects of Anthocyanin-Rich Roselle (Hibiscus sabdariffa L.) Extract on the Growth, Intestinal Histomorphology, Blood Biochemical Parameters, and the Immune Status of Broiler Chickens," Antioxidants, vol. 11, no. 3, Mar. 2022, doi: 10.3390/antiox11030544.

N. O. Eddy, B. I. Ita, S. N. Dodo, and E. D. Paul, "Inhibitive and adsorption properties of ethanol extract of Hibiscus sabdariffa calyx for the corrosion of mild steel in 0.1 M HCl," Green Chem. Lett. Rev., vol. 5, no. 1, pp. 43–53, 2012, doi: 10.1080/17518253.2011.578589.

M. E. Taghavizadeh Yazdi et al., "Gum tragacanth (Gt): A versatile biocompatible material beyond borders," Molecules, vol. 26, no. 6, Mar. 2021, doi: 10.3390/molecules26061510.

V. Rakić and N. Poklar Ulrih, "Influence of pH on color variation and stability of cyanidin and cyanidin 3-O-β-glucopyranoside in aqueous solution," CYTA - J. Food, vol. 19, no. 1, pp. 174–182, 2021, doi: 10.1080/19476337.2021.1874539.

M. . Koulani, R. Jamei, and A. . Marjani, "Influence of sucrose and high temperature on grape anthocyanin stability and furfural formation," J. Food Saf. Hyg., vol. 2, no. 3, pp. 54–62, 2016, [Online]. Available: http://jfsh.tums.ac.ir.

A. Fareed, H. Zaffar, M. Bilal, J. Hussain, C. Jackson, and T. A. Naqvi, "Decolorization of azo dyes by a novel aerobic bacterial strain Bacillus cereus strain ROC," PLoS One, vol. 17, 6 June, 2022, doi: 10.1371/journal.pone.0269559.

Ö. Karadaş, İ. Yılmaz, and Ü. Geçgel, "Determination of physicochemical properties of irradiated sumac (Rhus coriaria L.) fruit oils," Radiat. Phys. Chem., vol. 198, pp. 1–6, 2022, doi: 10.1016/j.radphyschem.2022.110210.

N. Tena, J. Martín, and A. G. Asuero, "State of the art of anthocyanins: Antioxidant activity, sources, bioavailability, and therapeutic effect in human health," Antioxidants, vol. 9, no. 5. MDPI, May 01, 2020, doi: 10.3390/antiox9050451.

Y.-K. Choong, N. S. A. Mohd Yousof, J. A. Jamal, and M. Isa Wasiman, "Determination of anthocyanin content in two varieties of Hibiscus Sabdariffa from Selangor, Malaysia using a combination of chromatography and spectroscopy," J. Plant Sci. Phytopathol., vol. 3, no. 2, pp. 067–075, 2019, doi: 10.29328/journal.jpsp.1001034.




DOI: http://dx.doi.org/10.18517/ijaseit.13.5.18750

Refbacks

  • There are currently no refbacks.


Published by INSIGHT - Indonesian Society for Knowledge and Human Development