Detection of Transposon Gene in Lurik Peanuts (Arachis hypogaea var. lurik L.) with AhMITEs Analysis

Ikhsanudin Nur Rosyidi, Adib Fakhruddin Yusuf, Mizan Sahroni, Budi Setiadi Daryono


Peanut (Arachis hypogaea L.) is one of the leading commodities in Indonesia that are consistently growing with high demand. However, its productivity in the current state is relatively few, thus causing dependency on imported products. Developing new varieties is one of the many solutions to these problems. Lurik peanuts are superior local varieties to any other peanuts in terms of productivity and disease resistance. Seed with the purple pattern is this cultivar's special characteristic and main attraction. This study aimed to identify and verify the activity of transposon genes in the seed pattern of Lurik peanuts. This research method was carried out by gene detection and sequencing analysis using PCR-AhMITEs (Arachis hypogaea Miniature Inverted Transposable Elements). The study used the Garuda variety as a comparison due to the absence of seed patterns, and it is a superior variety widely cultivated in Indonesia. Four types of primers used in this study were AhMITE1, AhTE0357, AhTE0391, and AhTE1317. The results revealed that the four primers had a linear relationship that could distinguish Lurik peanuts and Garuda peanuts based on the presence of transposon genes. The sequencing results confirmed that the detected genes were transposons from peanuts, located on chromosome 5 (Arahy.5), chromosome 9 (Arahy.9), chromosome 14 (Arahy.14), and chromosome 19 (Arahy.19). Based on the results of the study, the pattern on Lurik peanuts is an expression of the transposon gene activity.


Lurik peanuts; Garuda peanuts; Arachis hypgaea; transposon; AhMITEs

Full Text:



I. N. Rosyidi and B. S. Daryono, “Phenotypic characters and genetic variations of lurik peanuts (Arachis hypogaea l. var. lurikensis) with inter simple sequence repeat,” Biodiversitas, vol. 21, no. 2, pp. 629–635, Feb. 2020, doi: 10.13057/biodiv/d210227.

M. C. Stitzer, S. N. Anderson, N. M. Springer, and J. RossIbarra, “The genomic ecosystem of transposable elements in maize,” PLoS Genet, vol. 17, no. 10, Oct. 2021, doi: 10.1371/journal.pgen.1009768.

S. N. Anderson, M. C. Stitzer, P. Zhou, J. Ross-Ibarra, C. D. Hirsch, and N. M. Springer, “Dynamic patterns of transcript abundance of transposable element families in maize,” G3: Genes, Genomes, Genetics, vol. 9, no. 11, pp. 3673–3682, Nov. 2019, doi: 10.1534/g3.119.400431.

F. Pourrajab and S. Hekmatimoghaddam, “Transposable elements, contributors in the evolution of organisms (from an arms race to a source of raw materials),” Heliyon, vol. 7, no. 1. Elsevier Ltd, Jan. 01, 2021. doi: 10.1016/j.heliyon.2021.e06029.

N. Colonna Romano and L. Fanti, “Transposable Elements: Major Players in Shaping Genomic and Evolutionary Patterns,” Cells, vol. 11, no. 6. MDPI, Mar. 01, 2022. doi: 10.3390/cells11061048.

C. Mhiri, F. Borges, and M. A. Grandbastien, “Specificities and Dynamics of Transposable Elements in Land Plants,” Biology, vol. 11, no. 4. MDPI, Apr. 01, 2022. doi: 10.3390/biology11040488.

Y. E. Arvas, M. M. Abed, Q. A. Zaki, Kocacalişkan, and E. K. Haji, “The Potential Role of Transposable Elements as Molecular Markers,” in IOP Conference Series: Earth and Environmental Science, May 2021, vol. 761, no. 1. doi: 10.1088/1755-1315/761/1/012031.

S. N. Nayak, V. Hebbal, P. Bharati, H. L. Nadaf, G. K. Naidu, and R. S. Bhat, “Profiling of Nutraceuticals and Proximates in Peanut Genotypes Differing for Seed Coat Color and Seed Size,” Front Nutr, vol. 7, Apr. 2020, doi: 10.3389/fnut.2020.00045.

K. Shirasawaet al., “Characterization of active miniature inverted-repeat transposable elements in the peanut genome,” Theoretical and Applied Genetics, vol. 124, no. 8, pp. 1429–1438, May 2012, doi: 10.1007/s00122-012-1798-6.

Y. Tang et al., “Identification and characterization of transposable element AhMITE1 in the genomes of cultivated and two wild peanuts,” BMC Genomics, vol. 23, no. 1, Dec. 2022, doi: 10.1186/s12864-022-08732-0.

C. Wang et al., “Combining ability for main quality traits in peanut (Arachis hypogaea L.),” Oil Crop Science, vol. 6, no. 4, pp. 175–179, Oct. 2021, doi: 10.1016/j.ocsci.2021.10.005.

J. N. Wells and C. Feschotte, “A Field Guide to Eukaryotic Transposable Elements,” Annual Review of Genetics, vol. 54. Annual Reviews Inc., pp. 539–561, Nov. 23, 2020. doi: 10.1146/annurev-genet-040620-022145.

L. Lin, A. Sharma, and Q. Yu, “Recent amplification of microsatellite-associated miniature inverted-repeat transposable elements in the pineapple genome,” BMC Plant Biol, vol. 21, no. 1, Dec. 2021, doi: 10.1186/s12870-021-03194-0.

R. A. Gill et al., “On the Role of Transposable Elements in the Regulation of Gene Expression and Subgenomic Interactions in Crop Genomes,” CRC Crit Rev Plant Sci, vol. 40, no. 2, pp. 157–189, 2021, doi: 10.1080/07352689.2021.1920731.

A. Macko-Podgórni, G. Machaj, and D. Grzebelus, “A global landscape of miniature inverted-repeat transposable elements in the carrot genome,” Genes (Basel), vol. 12, no. 6, Jun. 2021, doi: 10.3390/genes12060859.

Y. J. Jeon, Y. H. Shin, S. J. Cheon, and Y. D. Park, “Identification and Characterization of PTE-2, a Stowaway-like MITE Activated in Transgenic Chinese Cabbage Lines,” Genes (Basel), vol. 13, no. 7, Jul. 2022, doi: 10.3390/genes13071222.

S. Perumal et al., “Characterization of B-Genome Specific High Copy hAT MITE Families in Brassica nigra Genome,” Front Plant Sci, vol. 11, Jul. 2020, doi: 10.3389/fpls.2020.01104.

S. Dai et al., “Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L.,” Oil Crop Science, vol. 6, no. 1, pp. 28–34, Mar. 2021, doi: 10.1016/j.ocsci.2021.03.004.

Y. Tang et al., “Identification of an active miniature inverted-repeat transposable element mJing in rice,” Plant Journal, vol. 98, no. 4, pp. 639–653, May 2019, doi: 10.1111/tpj.14260.

A. Bhat et al., “Role of Transposable Elements in Genome Stability: Implications for Health and Disease,” International Journal of Molecular Sciences, vol. 23, no. 14. MDPI, Jul. 01, 2022. doi: 10.3390/ijms23147802.

J. Wang, N. Lu, F. Yi, and Y. Xiao, “Identification of Transposable Elements in Conifer and Their Potential Application in Breeding,” Evolutionary Bioinformatics, vol. 16. SAGE Publications Ltd, 2020. doi: 10.1177/1176934320930263.

D. Gao et al., “ValSten: a new wild species derived allotetraploid for increasing genetic diversity of the peanut crop (Arachis hypogaea L.),” Genet Resour Crop Evol, vol. 68, no. 4, pp. 1471–1485, Apr. 2021, doi: 10.1007/s10722-020-01076-2.

D. J. Bertioliet al., “The genome sequence of segmental allotetraploid peanut Arachis hypogaea,” Nat Genet, vol. 51, no. 5, pp. 877–884, May 2019, doi: 10.1038/s41588-019-0405-z.

W. Zhuang et al., “The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication,” Nat Genet, vol. 51, no. 5, pp. 865–876, May 2019, doi: 10.1038/s41588-019-0402-2.

V. katesh, A. G. Vijaykumar, B. N. Motagi, and R. S. Bhat, “Single Marker Analysis Using Transposon Specific Markers (AhMITE1) for Yield, Foliar Disease Resistance and Oil Quality in a Mutant Population of Groundnut (Arachis hypogaea L.),” Int J CurrMicrobiol Appl Sci, vol. 8, no. 03, pp. 2376–2385, Mar. 2019, doi: 10.20546/ijcmas.2019.803.281.

G. Kubra et al., “Expression Characterization of Flavonoid Biosynthetic Pathway Genes and Transcription Factors in Peanut Under Water Deficit Conditions,” Front Plant Sci, vol. 12, Jun. 2021, doi: 10.3389/fpls.2021.680368.

Q. Xueet al., “Transcriptome and Metabolome Analysis Unveil Anthocyanin Metabolism in Pink and Red Testa of Peanut (Arachis hypogaea L.),” Int J Genomics, vol. 2021, 2021, doi: 10.1155/2021/5883901.

H. Speer, N. M. D’Cunha, N. I. Alexopoulos, A. J. McKune, and N. Naumovski, “Anthocyanins and human health—a focus on oxidative stress, inflammation and disease,” Antioxidants, vol. 9, no. 5. MDPI, Apr. 01, 2020. doi: 10.3390/antiox9050366.

B. Salehi et al., “The Therapeutic Potential of Anthocyanins: Current Approaches Based on Their Molecular Mechanism of Action,” Frontiers in Pharmacology, vol. 11. Frontiers Media S.A., Aug. 26, 2020. doi: 10.3389/fphar.2020.01300.

J. Liu et al., “Anthocyanins: Promising natural products with diverse pharmacological activities,” Molecules, vol. 26, no. 13, Jun. 2021, doi: 10.3390/molecules26133807.



  • There are currently no refbacks.

Published by INSIGHT - Indonesian Society for Knowledge and Human Development