Phytochemical Screening of Tacca Plant (Tacca leontopetaloides L.) Ethanol Extract Using Spectrophotometry UV-VIS Method

Sartika Syafi, Bambang Pujiasmanto, Edi Purwanto, Venty Suryanti


Phytochemical screening is a general approach to medicinal plants and is a local knowledge that exists in the community. Tacca (Tacca lentopetaloides L.) is a traditional plant with criteria as raw material for traditional medicine, but most people do not know the benefits of these plants. Phytochemical screening tests have been carried out on the ethanol extract of the taka plant by soaking for 3 days. This Study aims to determine the phytochemical compounds found in plant organs such as leaves, stems and tubers using spectrophotometric UV-vis methods and quantitative methods. The testing mechanism uses the Tacca plant's leaves, stems and tubers. Screening phytochemicals of ethanol extract included tests for flavonoids, alkaloids, tannins, phenols, and saponins. From the results of the Study, it can be seen that the location of the Tacca plant grows very significantly affects phytochemical compounds. From the three research locations, Gorango village has higher yields, namely the tubers that have a number of alkaloid compounds around 2,496.35 mg/kg and saponins (4,203.32 mg/kg), followed by flavonoids (7.20), tannins. (12.74) and phenol (4.08), followed by the villages of Bebsili and Gurua. This indicates that the Tacca plant contains phytochemical compounds, including alkaloids, flavonoids, saponins, phenols and tannins which have properties such as pain relievers, anticancer, antibacterial, anti-viral, so that the Tacca plant can be used as recommendation material as a mixture of medicinal ingredients in world of medicine.


Screening phytochemical; tacca plants; spectrophotometric UV-vis; ethanol extract.

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Maleš, I., Pedisić, S., Zorić, Z., Elez-Garofulić, I., Repajić, M., You, L., and Dragović-Uzelac, V. "The medicinaal and aromatic plants as ingredients in functional beverage productiion". Journal of Functional Foods, 96, 105210. 2022.

Sun, P., Zhao, W., Wang, Q., Chen, L., Sun, K., Zhan, Z., and Wang, J. "Chemical diversity, biological activities aand Traditional uses of and important Chinese herb Sophora". Phytomedicine, 154054. 2022.

Ma, D., Wang, S., Shi, Y., Ni, S., Tang, M., & Xu, A." The development of traditional Chines medicine". Journal of Traditional Chinese Medical Sciences, 8, S1-S9. 2021.

Wang, Y. J., Li, Y. X., Li, S., He, W., Wang, Z. R., Zhan, T. P., and Zeng, X. X. "Progress in traditional Chinese medicine and natural extracts for the treatment of lupus nephritis". Biomedicine & Pharmacotherapy, 149, 112799. 2022.

Maleš, I., Pedisić, S., Zorić, Z., Elez-Garofulić, I., Repajić, M., You, L., and Dragović-Uzelac, V. "The medicinal and aromatic plants as ingredients in functional beverage production". Journal of Functional Foods, 96, 105210. 2022.

Khlibsuwan, R., Tansena, W., & Pongjanyakul, T. "Modification of alginate bead using gelatinized aand ungelatinized arrowroot (Tacca leontopetaloides L. Kuntze) starch for drug delivery". International journal of biological macromolecules, 118, 683-692. 2018.

S. F. K. Agung, D. Ramdhani, and R. Mustarichie, "Syzygium polyanthum (weight) Walp Leaves Extracts as the Antibacterial Agent for Staphylococcus Aureus," World Journal of Pharmaceutical Res, vol. 9, no.7, pp. 2463-2468. Jun 2020.

Li, Y., Cao, S. Y., Lin, S. J., Zhang, J. R., Gan, R. Y., & Li, H. B. "Polyphenolic profile and antioxidant capacity of extracts from Gordonia axillaris fruits". Antioxidants, 8(6), 150. 2019.

Syafi, S., Pujiasmanto, B., Purwanto, E., & Suryanti, V. "Identification of morphology and analysis of Tacca (Tacca Leontopetaloides L.) medicine plant grown in North Maluku, Indonesia". In AIP Conference Proceedings (Vol. 2120, No. 1, p. 030021). July 2019.

Chanwitheesuk, A., Teerawutgulrag, A., & Rakariyatham, N. "Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand". Food chemistry, 92(3), 491-497. 2005.

Nurzaman, M., Abadi, S. A., Setiawati, T., & Mutaqin, A. Z. "Characterization of the phytochemical and chlorophyll content as well as the morphology and anatomy of the Rhizophoraceae family in the mangrove forest in Bulaksetra, Pangandaran". In AIP conference proceedings (Vol. 2021, No. 1, p. 030015). AIP Publishing LLC. October, 2018.

Arbiastutie, Y., Diba, F., & Masriani, M. "Ethnobotanical and ecological studies of medicinal plants in a mangrove forest in Mempawah District, West Kalimantan, Indonesia". Biodiversitas Journal of Biological Diversity, 22(6). 2021.

Bigoniya, P., Singh, C. S., & Srivastava, B. "Pharmacognostical and physico-chemical standardization of Syzygium cumini and Azadirachta indica seed". Asian Pacific Journal of Tropical Biomedicine, 2(1), S290-S295. 2012.

Wijayanti, T., Munawwaroh, A., & Hidayati, N. "Characterization of phytochemical compounds (qualitative and quantitative) in the endophytic fungi the bark of the Duwet plant". Jurnal Biologi Tropis, 22(2), 610-618. 2022.

Srećković, N., Stanković, J. S. K., Matić, S., Mihailović, N. R., Imbimbo, P., Monti, D. M., and Mihailović, V. "Lythrum salicaria L.(Lythraceae) as a promising source of phenolic compounds in the modulation of oxidative stress: Comparison between aerial parts and root extracts:. Industrial Crops and Products, 155, 112781. 2020.

M. Walean, R. Melpin, M. Rondonuwu, and K. F. Pinontoan, "Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract." Biodiversitas Journal of Biologycal Diversity. vol. 21, no. 6. pp. 2377-2382, Jun. 2020.

Elham, A., Arken, M., Kalimanjan, G., Arkin, A., and Iminjan, M." A review of the phytochemical, pharmacological, pharmacokinetic, and toxicological evaluation of Quercus Infectoria galls". Journal of Ethnopharmacology, 273, 113592. 2021.

Khan, B. M., Qiu, H. M., Wang, X. F., Liu, Z. Y., Zhang, J. Y., Guo, Y. J., and Cheong, K. L. "Physicochemical characterization of Gracilaria chouae sulfated polysaccharides and their antioxidant potential". International journal of biological macromolecules, 134, 255-261. 2019.

Tang, L., Luo, X., Wang, M., Wang, Z., Guo, J., Kong, F., and Bi, Y. "Synthesis, characterization, in vitro antioxidant and hypoglycemic activities of selenium nanoparticles decorated with polysaccharides of Gracilaria lemaneiformis". International Journal of Biological Macromolecules, 193, 923-932. 2021.

Martínez-Ceja, A., Romero-Estrada, A., Columba-Palomares, M. C., Hurtado-Díaz, I., Alvarez, L., Teta-Talixtacta, R., and Bernabé-Antonio, A. "Anti-inflammatory, antibacterial and antioxidant activity of leaf and cell cultures extracts of Randia aculeata L. and its chemical components by GC-MS". South African Journal of Botany, 144, 206-218. 2022.

Silva, B., Biluca, F. C., Gonzaga, L. V., Fett, R., Dalmarco, E. M., Caon, T., and Costa, A. C. O. "In vitro anti-inflammatory properties of honey flavonoids: A review". Food Research International, 141, 110086. 2021.

Solihah, I., Wulandari, W., Rasyid, R. S. P., and Suciati, T." Study on the anti-inflammatory properties of Karamunting (Rhodomyrtustomentosa (Aiton) Hassk.) leaf extracts". In Journal of Physics: Conference Series (Vol. 1282, No. 1, p. 012087). IOP Publishing. July, 2019.

Qiao, Q., Dua, Y., & Xie, L. "Research advances of erianin: source, production, biological activities and pharmacological properties". Pharmacological Research-Modern Chinese Medicine, 100059. 2022.

Ita, B. N., and Eduok, S. I. "In vitro antioxidant and antifungal activities of Rhizophora racemosa GFW Mey. stem bark extracts". Scientific African, 15, e01091. 2022.

Rahim, A. A., Rocca, E., Steinmetz, J., Kassim, M. J., Adnan, R., & Ibrahim, M. S. "Mangrove tannins and their flavanoid monomers as alternative steel corrosion inhibitors in acidic medium". Corrosion science, 49(2), 402-417. 2007.

Kustiati, U., Wihadmadyatami, H., & Kusindarta, D. L. "Dataset of Phytochemical and secondary metabolite profiling of holy basil leaf (Ocimum sanctum Linn) ethanolic extract using spectrophotometry, thin layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance". Data in Brief, 40, 107774. 2022.

Nugraha, J., Sukmawati, A., Mulyawan, A. S., and Sugiyana, D. "Biduri (Calotropis gigantea) leaves extract as natural dyes and ultraviolet protector applied on silk fabric with an exhaust dyeing method". In IOP Conference Series: Materials Science and Engineering (Vol. 980, No. 1, p. 012021). IOP Publishing. December, 2020.

Kokilaramani, S., Narenkumar, J., AlSalhi, M. S., Devanesan, S., Obulisamy, P. K., Balagurunathan, R., and Rajasekar, A. "Evaluation of crude methanolic mangrove leaves extract for antibiofilm efficacy against biofilm-forming bacteria on a cooling tower wastewater system". Arabian Journal of Chemistry, 15(7), 103948. 2022.

Baky, M. H., Elsaid, M. B., & Farag, M. A. "Phytochemical and biological diversity of triterpenoid saponins from family Sapotaceae: A comprehensive review". Phytochemistry, 113345. 2022.

Najjar-Tabrizi, R., Javadi, A., Sharifan, A., Chew, K. W., Lay, C. H., Show, P. L., and Berenjian, A. "Hydrothermally extraction of saponin from Acanthophyllum glandulosum root–Physico-chemical characteristics and antibacterial activity evaluation". Biotechnology Reports, 27, e00507. 2020.

Wasser, S. P., Didukh, M., and Nevo, E. "Antitumor and immunomodulatory activities of medicinal mushroom polysaccharide and polysaccharide-protein complexes in animals and humans". Mycol Balcan, 2, 221-50. 2005.

Jisieike, C. F., & Betiku, E. "Rubber seed oil extraction: effects of solvent polarity, extraction time and solid-solvent ratio on its yield and quality". Biocatalysis and Agricultural Biotechnology, 24, 101522. 2020.



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