Growth and Physiological Study of Gamma-Induced M4 Black Rice in Stress Condition

Anjakasi Mar’atul Ula, Edi Purwanto, - Parjanto


Increased rice productivity can be achieved by producing high-yielding black rice seeds. One of the productions of superior seeds is by mutation induction using gamma-ray irradiation. Black rice has a useful content and will be produced when the plant grows in drought stress. This research aims to study the growth and physiological characteristics of black rice induced by gamma rays of 100 gray and 200 gray, as well as know the effect of drought stress on proline content, to obtain superior black rice seeds. The design was researched in the form of an experiment in the field without using a repeat sample. Black rice was planted as a screen house in Jati Village, Karanganyar. The treatment used 24 plant genotypes, namely, 10 genotypes of 100 gamma-ray irradiation and 2 control genotypes, as well as 10 genotypes of 200 gamma-ray irradiation and 2 genotypes of control. Physiological observation variables were plant height, plant dry weight, root dry weight, proline content, and leaf temperature. The results of the research on plant height growth with gamma ray irradiation resulted in shorter plant postures. Leaf temperature and proline content in plants treated with irradiation and drought stress had higher values than in control plants. M4-By-C and M4-Bt-C lines are prospective to be released as new high-yielding varieties because these lines have short stature and high proline content.


Irradiation; mutation seeds; drought stress

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M.S. Farooq, X. Wang, M. Uzair, H. Fatima, S. Fiaz, Z. Maqbool, O.U. Rehman," Recent trends in nitrogen cycle and eco-efficient nitrogen management strategies in aerobic rice system", J. Frontiers in Plant Science, vol.13, no.36, pp. 24, 2022, doi: 10.3389/fpls.2022.960641.

E. Abascal, L. Gómez-Coma, Ortiz, "Global diagnosis of nitrate pollution in groundwater and review of removal technologies", J. Sci Total Environ, vol.810, pp. 152233, 2022, doi: 10.1016/J.SCITOTENV.2021.15223.

M.A. Haque, R. Mia, S.T. Mahmud, M.A. Bakar, "Sustainable dyeing and functionalization of wool fabrics with black rice extract", J. Science Direct, vol. 7, no.1, pp. 10334, 2022, doi: 10.1016/j.resenv.2021.100045.

T.A. Urmi, M.M. Islam, K.N. Zumur, M.A. Abedin, M.M. Haque, "Combined Effect of Salicylic Acid and Proline Mitigates Drought Stress in Rice (Oryza sativa L.) through the Modulation of Physiological Attributes and Antioxidant Enzymes", J. MDPI, vol. 12, no.7, pp. 1-9, 2023. doi: 10.3390/antiox12071438.

S.M.N. Tourky, W.M. Shukry, M.A. Hossain, "Cobalt enhanced the drought-stress tolerance of rice (Oryza sativa L.) by mitigating the oxidative damage and enhancing yield attributes", J. Science Direct, vol. 159, no.5, pp. 197-207, 2023. doi: 10.1016/j.sajb.2023.05.035.

M. Sharma, M. Chaudhary, S.N. Raina, D. Sahoo, "Comparative analysis of the efficiency of seed protein profiles in assessing genetic variation and population structure among indigenous Manipur black rice cultivars", J. Springer, vol. 50, pp. 3365–3378, 2023, doi: 10.5897/AJB11.2178.

O. Herliana, I. Widiyawati, and S. N. Hadi, "The Effect of Stable Manure and Seedling Number on Growth and Yield of Black Rice (Oryza sativa L. Indica)," Caraka Tani J. Sustain. Agric., vol. 34, no. 1, pp. 13–21, 2019. doi: 10.20961/ carakatani.v34i1.27098.

E.Beyramizadeh, A. Arminian, A. Fazeli, "Evaluating the effect of gamma rays on Zamiifolia (Zamioculcas zamiifolia) plant in vitro and genetic diversity of the resulting genotypes using the ISSR marker", J. S Nature. vol. 13, no. 8308, pp 522-534, 2023, doi: 10.1038/s41598-023-35618-2.

S.N. Mizil, E.A.J. ElKaaby, M.Z.F. Al-Shammary, "In Vitro Effect of Mannitol Stress and γ-ray on Secondary Products From Golden Sunrise Cherry Tomato Callus", J. Ibn Al-Haitham Journal for Pure and Applied Sciences, vol.36, no. 3, 2023, doi: 10.30526/36.3.3117.

İ.H. Çerçi, Ö.F. Durusoy, K.N. Çerçi, "The Effects of Shriveling up Waste Watermelons in Different Solar Drying Systems on Withering and Relevant Microbial Parameters", J. Scielo Brazil, vol. 1, no. 66, 2023, doi: 10.1590/1678-4324-2023220861.

B. Adhikari, O.J. Olorunwa , S. Brazel , T.C. Barickman, "Impact of salt stress on physiology, leaf mass, and nutrient accumulation in romaine lettuce", J. Photosynthetica, vol. 61, no.3, pp. 342-353, 2023, doi: 10.32615/ps.2023.027.

L S Bates, RP Waldren, ID Teare, "Rapid Determination of Free Proline for Water-stress Studies". Plant and Soil, Vol 39(1), pp.205-207, 1973. doi: 10.1016/j.jinsphys .2022.104363.

S. Syafi, V. Suryanti, B. Pujiasmanto, and E. Purwanto, “Morphological Response of Takka Plant (Tacca leontopetaloides L.) as Traditional Medicine for Drought Stress,†in 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019), 2020, pp. 121–125. doi: 10.2991/aer.k.200325.024

N. Francis, R. Rajasekaran, "Gamma irradiation to induce beneficial mutants in proso millet (Panicum miliaceum L.): an underutilized food crop", J. Radiation Biology vol.1, pp. 1277-1288, 2022, doi: 10.1090/09553002,2022,20224292

A.J.S. Abaka, M.H.S. Al-Asadi, N.Y. Abdel, "Effect of Cultivar and Spraying With The Amino Acid Phenylalanine on The Yield of Black Rice Grains and Their Content of Some Anthocyanin Pigments", J.Annals of Forest Research, vol.66. no.1, pp.3278-3290, 2023, doi: 10.3390/jof9060611

O. Ahluwalia, P. C. Singh, and R. Bhatia, "A review on drought stress in plants: Implications, mitigation and the role of plant growth promoting rhizobacteria," Resour. Environ. Sustain., vol. 5, p. 100032, 2021. doi: 10.1016/j.resenv.2021.100032

R.K. Salgotra, B.S. Chauhan, "Ecophysiological Responses of Rice (Oryza sativa L.) to Drought and High Temperature", J. MDPI Agronomy, vol.13, no.7.pp. 1877, 2023, doi: 10.3390/agronomy13071877.

M.J. Zaghum, K. Ali, S. Teng, "Integrated Genetic and Omics Approaches for the Regulation of Nutritional Activities in Rice (Oryza sativa L.)", J. MDPI Agriculture, vol. 1,. No.11, pp. 1757, 2022, doi: 10.3390/agriculture12111757.

S.A.F. El-Okkiah, M.M. El-Afry, "Foliar spray of silica improved water stress tolerance in rice (Oryza sativa L.) cultivars", J.Frontiers in Plant Science, vol.13, no.1, 2022, doi: 10.3389/fpls.2022.935090.

R. Pipai, A. McNeill, M. Unkovich, M. Banabas," Biological nitrogen fixation by legume cover plants in oil palm plantations: calibration of the ureide technique and effects of plantation age and soil nitrat", J. Springer, vol.8, no.1, pp. 1-16, 2023, doi: 10.1007/s11104-023-06147-8.

B. Sharma, S. Tiwari, K.C. Kumawat," Nano-biofertilizers as bio-emerging strategies for sustainable agriculture development: Potentiality and their limitations", J. Science Direct, vol. 860, 2023, doi: 10.1016/j.scitotenv.2022.160476.

N.S. Shimoyama, "Targeted Mapping of Oryza sativa Chilling Tolerance Candidate Genes Using Multiple Stress-Relevant Quantitative Traits", Ph.D. dissertation, Faculty of the Graduate School, Marquette University, 2023.

H.F. Alharby,†Combined Role of Fe Nanoparticles (Fe NPs) and Staphylococcus aureus L. in the Alleviation of Chromium Stress in Rice Plantsâ€, J.MDPI Life, vol.12, no.3, pp. 338, 2022, doi: 10.1016/j.scitotenv.2022.160476.

R.S. Rea, M.R. Islam, M.M. Rahman, B. Nath, K. Mix, "Growth, Nutrient Accumulation, and Drought Tolerance in Crop Plants with Silicon Application: A Review", J.MDPI Sustainability, vol.14, no.8, pp.4525, 2022, doi: 10.3390/su14084525.

S. Shrestha, "Bioconversion of agricultural residues into value-added products by pectinase-producing bacteria and expression of pectinase gene in E. coli for biomass valorization", Ph.D. dissertation Department of Biotechnology, Lakehead University, 2023.

A. Thomas, R. Beena, G. Laksmi, K.B. Soni, S. Alex, "Changes in sucrose metabolic enzymes to water stress in contrasting rice genotypes", J. Science Direct, vol.5, 2022, doi: 10.1016/j.stress.2022.100088.

A. He, T. Zhao, Q. Yin, Y. Mu, Y. Wang," Effects of shading at different growth stages with various shading intensities on the grain yield and anthocyanin content of colored rice (Oryza sativa L.)", J. Science Direct,vol.283, no.108555, 2022, doi: 10.1016/j.fcr.2022.108555.

J. Singh, S. Kumar, "Evaluation of the DNDCv.CAN model for simulating greenhouse gas emissions under crop rotations that include winter cover crops", J.Csiro, vol.60, no.6, pp.534-546, 2021, doi; 10.1071/SR21075.

F. Sarsu, S. Penna, G.C. Nikalje, "Strategies for Screening Induced Mutants for Stress Tolerance", J.Springer, pp.151-176, 2023, doi; 10.1079/9781789249095.000.

W. Sadok, J. R. Lopez, and K. P. Smith, "Transpiration increases under high-temperature stress: Potential mechanisms, trade-offs and prospects for crop resilience in a warming world," Plant, Cell & Environ., vol. 44, no. 7, pp. 2102–2116, 2021. doi: 10.1111/pce.13970.



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