Stability of Amylase Crude Powder from Aspergillus awamori KT-11 at Different Storage Temperatures

Urip Perwitasari, Ario Betha Juanssilfero, Ade Andriani, Ruth Melliawati, - Fahrurrozi, Ahmad Fathoni, - Sulat, Mukhammad Asy’ari, - Yopi


Amylase is one kind of biotechnology product that has been used in many sector industries. Production of amylase powder from Aspergillus awamori KT-11 has been optimized successfully in the previous study. However, the stability of its activity, which is crucial in industrial application, has not yet been well studied. This study aimed to investigate the stability of amylase crude powder activity from A. awamori KT-11 at different storage temperatures. Amylase crude powder was stored at 4oC and 25oC. For the stability test, the crude powder with optimum storage temperature was diluted with phosphate buffer pH 4.8 and stored at 4oC, 25oC, and -20oC. The results showed that the activity of amylase crude powder was stable at 25 oC after nine months of storage with total activity of 2807 U/mL. On the other hand, the amylase extract liquid enzyme shows better activity when stored at 4 oC (with total activity 1000 U/mL after four-week storage time) than stored at temperatures 25oC or -20 oC. The findings also showed that crude amylase liquid extracts are not stored in a negative temperature environment. This is beneficial to enterprises because it lowers the cost of obtaining negative temperatures and saves energy.


Enzyme stability; Aspergillus awamori KT-11; storage temperature; extract liquid enzyme.

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