Development of Chemiluminescence Resonance Test System Using SiPM Front-end ASIC to Detect Na and K Ions in Urine

Hojong Chang, KyungDon Choi, Byunghun Han, Hyunduk Kim, Jaeho Ko, Gyuseong Cho


The importance of measure and control dietary salinity arises to prevent and control the disease. There are several methods to measure the dietary salinities from blood or urine. The blood test is an accurate but inconvenient method because patients need to be at hospitals and wait for a longer time. Urine can be collected at home, and the test is more convenient. A 24-hour urine test is more accurate than random urine (RU) may cause more human errors. For this reason, testing RU accuracy for application will increase the convenience of patients. A SiPM sensor system to measure Guanine-based chemiluminescence resonance test light was developed. An ASIC system was developed and packaged to a chip. A test board for the packaged chip was developed. In parallel, the layout of an ASIC chip was assembled with SiPM and tested in the dark chamber to understand the functionality. The ASIC chip was tested in various frequencies with the test board. At the target frequency, the ASIC chip achieved 870 gain, which is exceeding the goal of 100. The SiPM system was measured with an oscilloscope, and the output signal was as expected. The performance test was done at a very high frequency (100MHz) and achieved 80.5% detection compared to the original light source signal. The ASIC chip development was successful, and SiPM matched the specification of the target operation.


SiPM; RU; ASIC; Sodium; Potassium; Photon.

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Schmidt, C. W. (2017). Another side of a low-salt diet: Reductions in the salinity of drinking water may lower blood pressure. Environmental Health Perspectives.

Biggers, A., & Felman, A. (2019). MEDICAL-NEWS-TODAY_High blood pressure: What is high, symptoms, causes, and more.

Houston, M. C. (2011). The importance of potassium in managing hypertension. Current Hypertension Reports.

Grillo, A., Salvi, L., Coruzzi, P., Salvi, P., & Parati, G. (2019). Sodium intake and hypertension. Nutrients.

A. Stallings, M. Harrison, & M. Oria (2019). Dietary Reference Intakes for Sodium and Potassium. National Academies Press.

Shlezinger, M., Amitai, Y., Akriv, A., Gabay, H., Shechter, M., & Leventer-Roberts, M. (2018). Association between exposure to desalinated sea water and ischemic heart disease, diabetes mellitus and colorectal cancer; A population-based study in Israel. Environmental Research, 166, 620–627.

Naser, A. M., Rahman, M., Unicomb, L., Doza, S., Gazi, M. S., Alam, G. R., Karim, M. R., Uddin, M. N., Khan, G. K., Ahmed, K. M., Shamsudduha, M., Anand, S., Narayan, K. M. V, Chang, H. H., Luby, S. P., Gribble, M. O., & Clasen, T. F. (2019). Drinking Water Salinity, Urinary Macro-Mineral Excretions, and Blood Pressure in the Southwest Coastal Population of Bangladesh. Journal of the American Heart Association, 8(9).

Gianfredi, V., Bragazzi, N. L., Nucci, D., Villarini, M., & Moretti, M. (2017). Cardiovascular diseases and hard drinking waters: Implications from a systematic review with meta-analysis of case-control studies. Journal of Water and Health, 15(1), 31–40.

Koren, G., Shlezinger, M., Katz, R., Shalev, V., & Amitai, Y. (2017). Seawater desalination and serum magnesium concentrations in Israel. Journal of Water and Health, 15(2), 296–299.

Wu, J., Xun, P., Tang, Q., Cai, W., & He, K. (2017). Circulating magnesium levels and incidence of coronary heart diseases, hypertension, and type 2 diabetes mellitus: A meta-analysis of prospective cohort studies. Nutrition Journal, 16(1).

O'Connor, L. E., Hu, E. A., Steffen, L. M., Selvin, E., & Rebholz, C. M. (2020). Adherence to a Mediterranean style eating pattern and risk of diabetes in a US prospective cohort study. Nutrition and Diabetes, 10(1).

Seko, C., Odani, K., Wada, S., Yoshii, K., Segawa, H., Kitaoka, K., Masumoto, T., & Higashi, A. (2020). Characteristic dietary habits associated with high values of estimated 24-hours urinary sodium excretion and sodium-to-potassium ratio assessed by age group among the residents of a rural town in Japan. Clinical and Experimental Hypertension, 42(5), 449–459.

Hojong, C., Byunghun, H., Gyuseong, C., & Hyunduk, K. (2018). A Study on the Measurement of Aptamer in Urine Using SiPM. Journal of Advanced Research in Dynamical and Control Systems, 11-Special Issue, 1208–1210.

Kieneker, L. M., Gansevoort, R. T., Mukamal, K. J., De Boer, R. A., Navis, G., Bakker, S. J. L., & Joosten, M. M. (2014). Urinary potassium excretion and risk of developing hypertension: The prevention of renal and vascular end-stage disease study. Hypertension.

Jędrusik, P., Symonides, B., & Gaciong, Z. (2019). Performance of 24-hour urinary creatinine excretion-estimating equations in relation to measured 24-hour urinary creatinine excretion in hospitalized hypertensive patients. Scientific Reports, 9(1).

Jędrusik, P., Symonides, B., & Gaciong, Z. (2018). Comparison of three formulas to estimate 24-hour urinary sodium and potassium excretion in patients hospitalized in a hypertension unit. Journal of the American Society of Hypertension, 12(6), 457–469.

Koo, H., Lee, S.-G., & Kim, J.-H. (2015). Evaluation of random urine sodium and potassium compensated by creatinine as possible alternative markers for 24 hours urinary sodium and potassium excretion. Annals of Laboratory Medicine, 35(2), 238–241.

Park JE, Lee CH, Kim BS, Shin IH. (2010). Diagnostic usefulness of the random urine Na/K ratio in cirrhotic patients with ascites: a pilot study. Korean J Hepatol.

JÈ©drusik, P., Symonides, B., Wojciechowska, E., Gryglas, A., & Gaciong, Z. (2017). Diagnostic value of potassium level in a spot urine sample as an index of 24-hour urinary potassium excretion in unselected patients hospitalized in a hypertension unit. PLoS ONE

Lin, C., Piao, X., Pan, Q., Li, J., Shan, Z., & Teng, W. (2017). Spot urine potassium-creatinine ratio is a good alternative marker for 24-hour urine potassium in differential diagnosis of Hypokalemia. Medical Science Technology, 58, 137–144.

Ye, J., Li, N., Lu, Y., Cheng, J., & Xu, Y. (2017). A portable urine analyzer based on colorimetric detection. Analytical Methods.

Yang, R., Cheng, W., Chen, X., Qian, Q., Zhang, Q., Pan, Y., … Miao, P. (2018). Color Space Transformation-Based Smartphone Algorithm for Colorimetric Urinalysis. ACS Omega.

He, Y., Dong, K., Hu, Y., & Dong, T. (2017). Colorimetric recognition for urinalysis dipsticks based on quadratic discriminant analysis. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS.

Waters. (2016). How does high performance liquid chromatography work? : Waters.

Oka, K., & Furusawa, K. (2018). Electrophoresis. In Electrical Phenomena at Interfaces: Fundamentals Measurements, and Applications, Second Edition, Revised and Expanded.

Dong, J., Lian, J., Jin, Y., & Li, B. (2017). Guanine-based chemiluminescence resonance energy transfer biosensing platform for the specific assay of uracil-DNA glycosylase activity. Analytical Methods.

Chen, Z., Guo, J., Zhang, S., & Chen, L. (2013). A one-step electrochemical sensor for rapid detection of potassium ion based on structure-switching aptamer. Sensors and Actuators, B: Chemical.

Shi, C., Gu, H., & Ma, C. (2010). An aptamer-based fluorescent biosensor for potassium ion detection using a pyrene-labeled molecular beacon. Analytical Biochemistry.

Cho, S., Park, L., Chong, R., Kim, Y. T., & Lee, J. H. (2014). Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection. Biosensors and Bioelectronics.

(2018) Fast LED Light Pulser & SiPM.



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