Hydrolytic and Transglycolation Characteristics of Xanthomonas campestris and Bacillus megaterium in Several Substrates

Heny Herawati, Elmi Kamsiati, - Sunarmani, S Joni Munarso, Ina Supriyatna, Tri Aminingsih

Abstract


Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including Xanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by using several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity from Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of research included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and transglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were then analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the effect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis results showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%, respectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose, tapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity test's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an incubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by microorganisms.

Keywords


Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium.

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References


N. U. R. Miftahurrohmah, “Karakterisasi Isolat JS-1, Bakteri Alkalofilik Penghasil Siklodekstrin Glikosiltransferase ( CGTase ) dari Sumedang, Jawa Barat (Characterisation of JS-1 Isolate, an Alkalophylic Bacteria as Cyclodextrin Glycosyltransferase ( CGTase ) Producer from Su,†J. Ilmu Kefarmasian Indones., vol. 12, no. 2, pp. 216–221, 2014.

R. M. Haley, R. Gottardi, R. Langer, and M. J. Mitchell, “Cyclodextrins in drug delivery: applications in gene and combination therapy,†Drug Deliv. Transl. Res., vol. 10, no. 3, pp. 661–677, 2020.

F. Fatimah, Z. Aytac, T. Uyar, and G. R. Williams1, “An Exploration of Electrospun Fibers Containing Drug-Cyclodextrin Inclusion Complexes,†Pharm. Sci. Res., vol. 7, no. 1, pp. 34–44, 2020.

V. T. H. Doan et al., “Cyclodextrin-based nanoparticles encapsulating α-mangostin and their drug release behavior: potential carriers of α-mangostin for cancer therapy,†Polym. J., vol. 52, no. 4, pp. 457–466, 2020.

G. Yaşayan, B. Şatıroğlu Sert, E. Tatar, and İ. Küçükgüzel, “Fabrication and characterisation studies of cyclodextrin-based nanosponges for sulfamethoxazole delivery,†J. Incl. Phenom. Macrocycl. Chem., vol. 97, no. 3–4, pp. 175–186, 2020.

A. M. P. Nuñez et al., “Bio-based products control black rot (Xanthomonas campestris pv. campestris) and increase the nutraceutical and antioxidant components in kale,†Sci. Rep., vol. 8, no. 1, pp. 1–11, 2018.

S. T. Jones et al., “Modified cyclodextrins as broad-spectrum antivirals,†Sci. Adv., vol. 6, no. 5, 2020.

W. Tang, C. Zou, C. Da, Y. Cao, and H. Peng, “A review on the recent development of cyclodextrin-based materials used in oilfield applications,†Carbohydr. Polym., vol. 240, no. 8, p. 116321, 2020.

P. Hydrogels, T. Konieczny, P. Kurcok, and A. Domi, “α -Cyclodextrin-Based Polypseudorotaxane Hydrogels,†pp. 1–28, 2020.

L. Wang, S. Chen, and J. Wu, “Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli,†BMC Biotechnol., vol. 18, no. 1, pp. 1–9, 2018.

R. Handayani, M. Hawab, and J. Sulistyo, “Aktivitas Antioksidasi Polifenol Glikosida Hasil Reaksi Transglikosilasi Enzim CGTase dari Bacillus macerans Bacillus macerans,†vol. 4, pp. 18–22, 2002.

J. Sulistyo, “Assay for transglycosylation reaction of xanthomonas campestris on carbohydrate sources,†Int. J. Res. Agric. Food Sci., vol. 2, no. 6, pp. 1–7, 2014.

H. Hargono, B. Jos, and A. C. Kumoro, “Kinetics of the enzymatic hydrolysis of sweet cassava starch, bitter cassava, and gadung (Dioscorea hispida dennst) Flours at low temperature,†Bull. Chem. React. Eng. & Catal., vol. 12, no. 2, pp. 256–262, 2017.

X. Liu et al., “Cloning and characterisation of a novel amylopullulanase from Bacillus megaterium Y103 with transglycosylation activity,†Biotechnol. Lett., vol. 4, 2020.

N. K. Sura and L. Hiremath, “Isolation of Bacillus megaterium and its Commercial Importance,†Int. J. ChemTech Res., vol. 12, no. 04, pp. 30–36, 2019.

N. Tu, D. Vinh, and L. Thu, “Amylase producing bacillus megaterium T04 isolated in rach lang stream of Vietnam,†J. Appl. Pharm. Sci., vol. 5, no. 10, pp. 12–15, 2015.

M. J. Rojas, M. Amaral-Fonseca, R. Fernandez-Lafuente, R. de Lima Camargo Giordano, and P. W. Tardioli, “Recovery of starch from cassava bagasse for cyclodextrin production by sequential treatment with α-amylase and cyclodextrin glycosyltransferase,†Biocatal. Agric. Biotechnol., vol. 22, no. October, p. 101411, 2019.

T. Rostinawati and H. S. Lestari, “Skrining Bakteri Penghasil Enzim β -Siklodekstrin Transferase ( β -CGTase ) dari Tanah Jatinangor Glukosil,†J. Farm. Sains, dan Kesehat., vol. 3, no. 2, pp. 10–17, 2017.

R. Handayani, “Synthesis of flavonoid-a-glicoside through transglycosylation by enzyme and its activities as antioxidant,†Biodiversitas, J. Biol. Divers., vol. 9, no. 1, pp. 1–4, 2008.

T. Rostinawati, Y. C. Sumirtapura, and D. S. Retnoningrum, “Penentuan Aktivitas Siklisasi-β Siklodekstrin Glukosiltransferase A2-5a terhadap Pati Terlarut,†Fak. Farm. Univ. Padjadjaran, Jl. Raya Bandung-Sumedang KM 21 Jatinangor 45363, vol. 2, no. 1, pp. 1–5, 2016.

Y. Andriani, E. Rochima, R. Safitri, and S. R. Rahayuningsih, “Characterisation of Bacillus megaterium and Bacillus mycoides Bacteria as Probiotic Bacteria in Fish and Shrimp Feed,†KnE Life Sci., vol. 2, no. 6, p. 127, 2017.

M. Dubois, K. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith, “A colorimetric method for the determination of sugars,†Nature, vol. 168, no. 4265, p. 167, 1951.

S. Madonna, “Produksi Enzim Amilolitik dari Bacillus megaterium Menggunakan Variasi Kadar Pati Sagu (Metroxylon sp.),†Al-Kauniyah J. Biol., vol. 7, no. 1, pp. 22–27, 2016.

N. S. Djenar and E. W. S. Mulyono, “the Kinetics Both of Growth and Metabolite Production of X.Campestris Using of 4% Liquid Sugar Substrate From Cassava Hydrolisate,†J. Bahan Alam Terbarukan, vol. 6, no. 1, pp. 45–49, 2017.

H. Mahmud, A. Ismail, R. Abdul Rahim, K. O. Low, and R. Md. Illias, “Enhanced secretion of cyclodextrin glucanotransferase (CGTase) by Lactococcus lactis using heterologous signal peptides and optimisation of cultivation conditions,†J. Biotechnol., vol. 296, no. March, pp. 22–31, 2019.

S. Suhaimi et al., “Optimisation of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilised on hollow fiber membrane,†IOP Conf. Ser. Mater. Sci. Eng., vol. 736, no. 4, 2020.

C. M. Peixoto, S. L. de A. Coelho, and M. L. Cazetta, “Byproducts from cassava industry: alternative substrates for cyclodextrin glycosyltransferase production by alkalophilic Bacillus trypoxylicola SM–02,†An. Biol., no. 42, pp. 37–46, 2020.




DOI: http://dx.doi.org/10.18517/ijaseit.11.1.12403

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