Effect of Plant Growth Regulator on Growth, Yield and Catechin Content of Tea (Camellia sinensis (L.) O.Kuntze)

Intan Ratna Dewi Anjarsari, Jajang Sauman Hamdani, Cucu Suherman, Tati Nurmala, Erdiansyah Rezamela, Heri Syahrian Khomaeni, Vitria Puspitasari Rahadi


Catechins are one of the secondary metabolites contained in tea leaves. Cultivation practices such as pruning affect the shoot production and quality of tea yield. The use of plant growth regulators is a new breakthrough in tea plant engineering. This research aims to determine the interaction effect of type, height of pruning, and concentration of Benzil Amino Purine (BAP) and Gibberellin (GA) concentrations on tea plants' growth and catechin content after pruning. The experiment was conducted at Tea and Cinchona Research Centre Gambung in June 2018 until October 2018, with a split-plot design consisted of three factors as followed: main factor (a) type of pruning (clean and lung pruning); subfactor (b) pruning height (40 cm, 50 cm, and 60 cm); sub-sub factor (h) plant growth regulator (0 ppm, 60 ppm BAP, 50 ppm GA, 60 ppm BAP + 50 ppm GA). The result showed that 60 cm pruning and 60 ppm BAP in the third month after pruning significantly affected the chlorophyll content index (91,58I cci). There was an interaction between the pruning height of 60 cm and 50 ppm GA on fresh shoots weight per bush on the fourth plucking. Based on the response curve, at clean pruning, the optimum value at pruning height of 51.5 cm and 66,63 ppm BAP contributes to the catechin content of 1.88% while at lung pruning, the minimum value pruning height of 50.73 cm and 7.238 ppm with catechin content of 0.776%.


Benzil Amino Purine (BAP); catechins; gibberellin (GA); pruning.

Full Text:



Direktorat Jendral Tanaman Perkebunan. Pengaruh Iklim dan Kejadian La Nina dan Antisipasinya Terhadap Produksi Tanaman Teh Kementrian Pertanian. 2018.

(20I8). Indonesia Investment. Tea website.[Online].Available : https://www.indonesiainvestments.com/id/bisnis/komoditas/teh/item240

Y. Jiang , J. Hua, B. Wang, H.Yuan, and H. Ma, “Effects of Variety, Season, and Region on Theaflavins Content of Fermented Chinese Congou Black Tea, Journal of Food Quality. vol.1, pp. 1-9, Nov. 2018.

W.Y. Han, J. Huang, X. Li, Z. Li, G.J. Ahammed, P. Yan, and J.R. Stepp, “ Altitudinal effects on the quality of green tea in east China: a climate change perspective,â€, Eur Food Res Technol.vol. 243, pp. 323-330, July. 2016.

C. Zhang, C.L.C. Suen, C. Yang and S.Y. Quek, “Antioxidant capacity and major polyphenol composition of teas as affected by geographical location, plantation elevation and leaf grade,†Food Chem., vol. 244, pp. 109-119. 2018

S.L. Dalimoenthe, â€Tea (Camellia sinensis) Sustainable Technology,†in the Proceedings of the Sustainable Tea Industry Technical Meeting, 2006, p. 49-68

M. Wakamatsu, H. Yamanouchi, H. Sahara, T. Iwanaga, R. Kuroda, A. Yamamoto, Y. Minami, M. Sekijima, K. Yamada, and K. Kajiya, “ Catechin and caffeine contents in green tea at different harvest periods and their metabolism in miniature swine,†2019, Food. Sci. Nutr. vol. 7, pp 2769–2778, Aug. 2019.

S. Firouzi and F. Azarian, “Propellants of mechanical pruning and plucking of tea (a case of developing countries),†2019, Information Processing in Agriculture. vol. 6, pp. 454-461.

Q. Zhang, T. Li , Q. Wang, J. LeCompte, R. L. Harkess and G. Bi, “Screening Tea Cultivars for Novel Climates: Plant Growth and Leaf Quality of Camellia sinensis Cultivars Grown in Mississippi, United States.,†Front. Plant Sci., vol.11, pp. 1-12, March. 2020.

K.C. Mohale, A.T. Hintsa, M.A. Emanuel and F.N. Mudau, “Metabolic Profiling of Cultivated Bush Tea. (Athrixiaphylicoides DC.) in Response to Different Pruning Types,†Hortscience, vol. 53, pp. 993–998. July. 2018.

Leduc N, Roman H, Barbier F, Péron T, Huché-Thélier L, Lothier J, Demotes-Mainard S, S. S. (2014). Light signaling in bud outgrowth and branching in plants. Plants (Basel), 3(2), 223–250. https://doi.org/10.3390/plants3020223

Z.H. Lee, T. Hirakawa, N. Yamaguchi and T. Ito, “The Roles of Plant Hormones and Their Interactions with Regulatory Genes in Determining Meristem Activity (review),†J. Mol. Sci., vol. 20, pp. 1-19.Aug. 2019.

L. Zhang , C. Shen , J. Wei and W. Han, “Effects of Exogenous 6-Benzyladenine on Dwarfing, Shoot Branching, and Yield of Tea Plant (Camellia sinensis),†Hort. Science., vol. 53, pp. 651–655. Aug.2018.

I.R.D Anjarsari, J.S Hamdani, C. Suherman and T.Nurmala, “Effect of Pruning and Cytokinin Application on the Growth of Tea GMB 7 Clone,†Asian J. Plant Sci., vol. 18, pp. 110-116. June. 2019.

S. Rosniawaty, I.R.D Anjarsari & R. Sudirja,â€Application Of Cytokinins To Enhance Tea Plant Growth In The Lowlands,†Journal of Industrial and Beverage Crop,vol. 5, pp. 31-38. 2018.

Shu, K, W. Zhou, F. Chen, X. Luo and W. Yang, â€Abscisic Acid and Gibberellins Antagonistically Mediate Plant Develelopment and Abiotic Stress Responses,†Front. Plant Sci., vol.9 pp. 1-8. March.2018.

H. Li, T. Garcia, J., D. Latrasse, M. Benhamed, S. Schilderink, W. Zhou,â€Plant-specific histone deacetylases HDT1/2 regulate gibberellin 2-oxidase2 expression to control Arabidopsis root meristem cell number,â€Plant Cell., vol. 29, pp. 2183–2196. Sept. 2017.

B. Wang, H. Wei, Z. Xue, and W.H. Zhang, “Gibberellins regulate iron deficiency-response by influencing iron transport and translocation in rice seedlings (Oryza sativa),†Ann. Bot. 119, vol.pp. 945–956. 2017

Official Method of Analysis of the Association of Official Analytical Chemists (AOAC). The Association of Official Analitical Chemists Inc. 1984.

M.I.P. Atmaja, Shabri, H.S. Khomaini, H. Maulana, S. Harianto and D. Rohdiana, “Changes in chlorophyll and polyphenols content in Camellia sinensis var. sinensis at different stage of leaf maturity,†in IOP Conf. Series: Earth and Environmental Science, 2018, p.1-7.

P.J. Davies, The Plant Hormones: Their Nature, Occurrence, and Functions, Department of Plant Biology. Cornell University, Ithaca, New York 14853, USA, 2008

B. Wang, H. Wei, Z. Xue, and W.H. Zhang, “Gibberellins regulate iron deficiency-response by influencing iron transport and translocation in rice seedlings (Oryza sativa),†Ann. Bot. 119, vol.pp. 945–956. 2017

X. Zhuang, L. Jiang, “Chloroplast Degradation: Multiple Routes Into the Vacuole (mini Review Article),†Front. Plant Sci., vol.10 , pp. 1-8 , March. 2019.

L. Juez, K.A. Epyke, â€Plastids unleashed: their development and their integration in plant development,†International Journal of Developmental Biology , vol. 49, pp. 557-577, Nov. 2005.

D. Procházková, D. Haisel and N. Wilhelmova, “Antioxidant protection during ageing and senescence in chloroplasts of tobacco with modulated life span,†Cell Biochemistry and Function, vol. 26, pp. 582– 590. May. 2008.

Z.X. Li , W.J. Yang, G.J. Ahammed, C. Shen, P. Yan, X. Li and W.Y. Han, “Developmental changes in carbon and nitrogen metabolism affect tea quality in different leaf position,†Plant Physiol. Biochem, vol. 106 , pp. 327–335, Sept. 2016

J. Binenbaum, R. Weinstain, and S. Shani, “Gibberellin Localization and Transport in Plants,†Tend In Plant Science, vol.23,pp. 410-421, May. 2018.

C. Yue, H. Cao, X. Hao, J. Zeng, W. Qian, Y. Guo , N. Ye, Y. Yang , X. Wang, “Differential Expression of Gibberellin- And Abscisic Acid-Related Genes Implies Their Roles in the Bud Activity-Dormancy Transition of Tea Plants,†Plant Cell Rep., vol. 37, pp. 425-441.March. 2018.

K. Shu, W. Zhou, F. Chen, X. Luo and W. Yang, “Abscisic Acid and Gibberellins Antagonistically Mediate Plant Develelopment and Abiotic Stress Responses,†Front. Plant Sci., vol. 9, pp. 1-8. March. 2018.

H. Li, J.T. Garcia, D. Latrasse, M. Benhamed, S. Schilderink, W. Zhou, “Plant-specific histone deacetylases HDT1/2 regulate Gibberellin 2-Oxidase2 expression to control Arabidopsis root meristem cell number,†Plant Cell , vol. 29, pp. 2183–2196. Sept. 2017.

Y. Wen, S. Su, L.Ma & X. Wang, “Effects of gibberellic acid on photosynthesis and endogenous hormones of Camellia oleifera Abel in 1st and 6th leaves,†Journal of Forest Research, vol. 23, pp. 309-317. Sept. 2018.

N.G. Halford, Photosynthate partitioning.Plant Developmental Biology - Biotechnological Perspectives, eds Pua E. C., Davey M. R., editors. Berlin; Heidelberg: Springer, 2010

N. Subarna, “Economic analysis of the effect of fine, medium, and coarse plucking on the average of tea pluckers and plant productivity,†in 5st Tea Prosiding Simposium Bandung , 1990, p. 469-479.

S. Mitrowihardjo, “Catechin content and shoot yield of several superior tea clones (Camellia sinensis (L.) O. Kuntze) at different altitudes in Pagilaran plantation,†Dissertation, Program Studi Pemuliaan Tanaman. Fakultas Pertanian UGM, Yogyakarta. 2012.

A.R. Loveless, Principles of Plant Biology for Tropical Areas I, Gramedia Pustaka Utama: Jakarta.1991.

F.P. Gardner, R. B. Pearce, and R. L. Mitchell, Fisiologi Physiology of Crop Plants. Iowa State University Press. 1991.

Y.O. Koca, O. Erekul, “Changes of Dry Matter, Biomass and Relative Growth Rate with Different Phenological Stages of Corn, Agriculture and Agricultural Science Procedia, vol.10, pp. 67-75. 2016.

M. Farooq, A. Wahid, N. Kobayashi, D. Fujita, and S.M.A. Basra, “Plant drought stress: effects, mechanisms and management. A gronomy for Sustainable Development,â€vol. 29, pp.185-212. March. 2009.

R. Hajiboland, “Environmental and nutritional requirements for tea cultivation, Folia Hort., vol. 29, pp. 199-220. 2017.

A.L. Tariq and A.L. Reyaz, “Phytochemical analysis of Camellia sinensis leaves,†Int. J. of Drug Development and Research, vol. 4, pp. 311-316. 2012.

O.B. Aigbodion, I. Marcell, “Microbiological characteristics and phytochemical screening of some herbal teas in Nigeria,†European Scientific Journal, vol.9, pp. 149-160. June.2013.

N. Verma, S. Shukla, “Impact of various factors responsible for fluctuation in plant secondary metabolites, J. App. Res. Med. Arom. Plants, vol. 2, pp. 105–113. Dec.2015.

Giannakoula, “The effects of plant growth regulators on growth, yield, and phenolic profile of lentil plants,†Journal of Food Composition and Analysis, vol.28, pp. 46-53. Nov. 2015.

M.N. Fhatuwani, M.P. Nokwanda, ‘Effect of Seasonal Variations and Growth Conditions on Carbohydrate Partitioning in Different Organs and the Quality of Bush Tea,†HortScience, vol. 53, pp. 999-1005. July. 2018.

Y. Jiang, J. Hua, B. Wang, H. Yuan, and H. Ma, “Effects of Variety, Season, and Region on Theaflavins Content of Fermented Chinese Congou Black Tea,†Journal of Food Quality, vol.2018 pp. 1-9. Nov. 2018.

T. Samanta, J.N.R. Kotamreddy, B.C. Ghosh and A. Mitra, “ Changes in targeted metabolites, enzyme activities and transcripts at different developmental stages of tea leaves: a study for understanding the biochemical basis of tea shoot plucking,†Acta Physiol. Plant. vol. 39, pp. 1-11. 2017.

D.Costa, W.A.J.M, A.J.A Mohotti and M.A. Wijeratne,†Ecophysiology of tea (Review),†Braz. J. Plant Physiol., vol. 19, pp. 299-332, Oct. 2007.

B. Sriyadi, “Seleksi klon teh assamica unggul berpotensi hasil dan kadar katekin tinggi,†Jurnal Penelitian Teh dan Kina vol.15, pp. 1-10. 2012.

B. Martono, S. Falah, and E. Nurlaela,†Antioxidant Activities Of Gmb 7 Variety Of Tea At Different Altitude,†Journal of Industrial and Beverage Crops, vol. 3, pp. 53-60. March. 2017.

C. Zhang, C.L.C. Suen, C. Yang , S.Y. Quek, “Antioxidant capacity and major polyphenol composition of teas as affected by geographical location, plantation elevation and leaf grade,†Food Chem vol. 244, pp. 109-119. Apr. 2018.

S. Ahmed, T. Griffin, S.B. Cash, W.Y. Han, C. Matyas, C and C, Long, Global climate change, ecological stress, and tea production in Stress Physiology of Tea in the Face of Climate Change, Singapore: Springer, 2018.

C.R. Muoki, T.K. Maritim, W.A. Oluoch, S.M. Kaumya and J. K. Bore, “ Combating Climate Change in the Kenyan Tea Industry. 2018. (Review Article),†Front. Plant Sci., vol. 11, pp.1-10, March. 2020.

DOI: http://dx.doi.org/10.18517/ijaseit.12.1.11657


  • There are currently no refbacks.

Published by INSIGHT - Indonesian Society for Knowledge and Human Development