Low-Cost Media for in vitro Multiplication and Development of Protocorm Like Bodies (PLBs) of Eulophia graminea Orchid

A. Romeida, - Supanjani, S. S. Sinaga

Abstract


Eulophia graminea is a saprophytic orchid easily found in a high salinity environment along the coastline. A study aimed to explore low-cost media compositions enriched with organic matters for in vitro multiplication and development of protocorm-like bodies (PLBs) of E. graminea. This study consisted of two stages. The first stage was to determine the best basic media for in vitro PLBs multiplication by testing NPK 20-20-20, Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW). The second stage was to compare the development of PLBs of E. graminea using NPK 20-20-20 as the basic media, supplemented with three types of organic matters (Cavendish banana, potato and coconut water) and four organic matter concentrations (50,100, 150, 200 ml L-1), arranged under Factorial Completely Randomized Design with ten replications.  The results showed that orchid PLB of E. graminea could be planted on NPK medium with similar success in growth and development, in term of PLB number, PLB diameter, leaf number in plantlet, plantlet height, root number and root length, to those planted on MS medium.  Among complex organic growth supplements, including coconut water, potato, and banana, coconut water at a concentration of 57.17 – 98.67 ml L-1 resulted in the best multiplication of PLB, in term of the greatest number, PLB diameter, and fresh weight. Coconut water promotes the greatest conversion of PLB into plantlets of E. graminea at an optimum concentration of 91.63 – 102.89 ml L-1.  The price of using a complete fertilizer medium NPK is much lower than those of using MS medium, Knudson C medium, or Vacin and Went medium; this media was the most cost-effective media for mass micropropagation of E. graminea.

Keywords


orchid; Eulophia graminea; in vitro; organic matter; multiplication

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References


S. Diah, and T. Djarwaningsih. “Keanekaragaman Jenis-jenis Anggrek Kepulauan Karimunjawa”. Jurnal Tek. Ling. Vol. 10. No. 2. pp 167 – 172. 2009.

Y. Wijayani, Solichatun, and W. Mudyantini. “Pertumbuhan tunas dan struktur anatomi Protocorm Like Body anggrek Grammatophyllum scriptum (Lindl.) Bl. dengan pemberian Kinetin dan NAA”. J. Bioteknologi. Vol. 4. No. 2. pp 33-40. 2007

R. E. Mitchell. “An Exotic Orchid. Eulophia graminea. Invades Charlotte County”. Proc. Fla. State Hort. Soc. Vol. 126. pp 271–272. 2013.

A. Romeida, S. H. Sutjahjo, A. Purwito, D. Sukma, and Rustikawati. “Variasi Genetik Mutan Anggrek Spathoglottis plicata Blume. Berdasarkan Marker ISSR”. J. Agron. Indonesia Vol. 40. No. 3. pp 218 – 224. 2012.

M.A. Baque, Y. K. Shin, T. Elshmari, E. J. Lee, and K.Y. Paek. “Effect of light quality, sucrose and coconut water concentration on the microporpagation of Calanthe hybrids (‘Bukduseong’ × ‘Hyesung’ and ‘Chunkwang’ × ‘Hyesung’)”. AJCS. Vol. 5. No. 10. pp 1247-1254. 2013.

T. Murashige, and F. Skoog. “A revised medium for rapid growth and bioassays with tobacco tissue cultures”. Physiol. Plant. Vol. 15. pp 473-497. 1962.

S. J. Nahar, S.H. Kazuhiko, L. H. Chieh, and N. Kaewjampa. “Effect of plant growth regulators on organogenesis in protocorm-like body (PLBs) of Cymbidium dayanum in vitro”. ARPN Journal of Agricultural and Biological Science. Vol. 6. No. 6. pp 28-33. 2012

R. Poobathy, N. Izwa, A. L. Julkifle, and S. Subramaniam. “Cryopreservation of Dendrobium sonia-28 using an alternative method of PVS2 droplet freezing”. Emir. J. Food Agric. Vol. 25. No.7. pp 531-538. 2013.

R. Ernst. “Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orhidaceae)”. Plant Cell Tiss. Organ Cult. Vol. 39. pp 273-275. 1994.

R. Murdad, K.S. Hwa, S. C. Khen, A. M. Latip, A. Z. Aziz, and R. Ripin. “High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique”. Scientia Horticulturae. Vol. 111. pp 73–79. 2006.

L. Knudson. “A new nutrient solution for germination of orchid seed”. Am. Orchid Soc. Bull. Vol. 15. pp 214-217. 1946.

G. Jakobsone. “Morphogenesis of wild orchid Dactylorhiza fuchsia in tissue culture”. Acta Universitatis Latviensis. Vol. 745. pp 17-23. 2008.

K. Obsuwan, and C. Thepsithar. “An Effect of Organic Supplements on Stimulating Growth of Vanda and Mokara Seedlings in Tissue Culture”. Int. J. Biol. Biomolec. Agric. Food Biotechnol. Eng. Vol. 8 No. 7. pp. 696-698. 2014.

P. Gnasekaran, R. Poobathy, M. Mahmood, M.R. Samian, and S. Subramaniam, “Effects of complex organic additives on improving the growth of PLBs of Vanda Kasem’s Delight”, Aus. J. Crop Sci., Vol. 6. No. 8. pp. 1245–1248. 2012.

A. Romeida, D. w. Ganefianti, Rustikawati, and Marlin. “Foliar fertilizer aplication for inducing rapid and uniform flowering on Spahoglottis plicata blume. Var. Alba orchid”. In Proc. The 3rd International Symposium for Sustainable Humanosphere (ISSH) A Forum of Humanosphere Science School (HSS) Bengkulu. pp 213-219. 2013.

G. Muawanah. “Penggunaan Pupuk Hyponex. Ekstrak Tomat dan Ekstrak Pisang dalam Perbanyakan dan Perbesaran Plantlet Anggrek Dendrobium Secara In Vitro”. Skripsi. Program Studi Hortikultura. Fakultas Pertanian. Institut Pertanian Bogor. Bogor Indonesia. 2005

J.F.P. Katuuk. “Aplikasi mikropogasi anggrek macan (Grammatohyllum scriptum)”. Jurnal Penelitian IKIP Manado Vol. 1. No. 4. pp 290-298. 2000.

Kasutjianingati, and I. Rudi. “Media Alternative Perbanyakan In-Vitro Anggrek Bulan (Phalaenopsis amabilis)”. Jurnal Agroteknos. Vol. 3. No. 3. pp 184-189. 2013.

Y. Bey, W. Syafii, and Sutrisna. “Pengaruh pemberian giberelin (GA3) dan air kelapa terhadap perkecambahan bahan biji anggrek bulan (Phalaenopsis amabilis BL) secara in vitro”. Jurnal Biogenesis. Vol. 2. No. 2. pp 41-46. 2006.

E. Vacin, and F.W. Went. “Some pH changes in nutrient solutions”. Bot. Gaz. Vol. 110. No. 4. Pp. 605-613. 1949.

Mukarlina, A. Listiawati, and S. Mulyani. “The effect of coconut water and Naphthalene Acetic Acid (NAA) application on the in vitro growth of Paraphalaeonopsis serpentilingua from West Kalimantan”. Bioscience. Vol. 2. No. 2. pp 62-66. 2010.

S. Tuhuteru, M. L. Hehanussa, and S.H.T. Raharjo. “Pertumbuhan dan perkembangan anggrek Dendrobium anosmum pada media kultur in vitro dengan beberapa konsentrasi Air Kelapa”. Jurnal Agrologia. Vol. 1. No. 1. pp 1-12. 2012.

J. W. H. Yong, L. G. Yong, F. Ng. Yan, and N. T. Swee. “The Chemical Composition and Biological Properties of Coconut (Cocos nucifera L.) Water” Molecules. Vol. 14. pp 5144-5164. 2009.

A. P. Prades, M. D. N. Diop, and J. P. Pain. “Coconut water uses, composition and properties: a review”. Fruits. Vol. 67. pp 87-107. 2012.

A. Sucandra, F. Silvina, and A. E. Yulia. “Uji pemberian beberapa konsentrasi glisin pada media Vacin and went (vw) terhadap pertumbuhan plantlet anggrek (Dendrobium sp) secara in vitro”. Jurnal Faperta Vol 2. No. 1. pp 1-9. 2015.

S. A. Asghar, T. Ahmad, I. A. Hafiz, and M. Yaseen. “In vitro propagation of orchid (Dendrobium nobile) var. Emma white”. Afr. J. Biotech. Vol. 10. No. 16. pp 3097- 3103. 2011.

E. Fibrianty. “Induksi Protocorm-like Bodies (PLBs) dan Karakteristik Molekuler Populasi F2 Anggrek Phalaenopsis”. In Tesis. Sekolah Pascasarjana. Institut Pertanian Bogor. Bogor Indonesia. 2013.

S. Akter, K. M. Nasiruddin, and K. Hossain. “Effects of different media and organic additives interaction on in vitro regeneration of Dendrobium orchid”. J. Agric. Rural Dev. Vol. 6. No. 1. pp 69-74. 2008.

P. Shekarriz, M. Kafi, S. D. Deilamy, and M. Mirmasoumi. “Coconut Water and Peptone Improve Seed Germination and Protocorm Like Body Formation of Hybrid Phalaenopsis”. Agric. Sci. Dev. Vol. 3. No.10. pp 317-322. 2014.

S. Kaur, and K.K. Bhutani. “Organic growth-supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw”. Hort. Sci. (Prague). Vol. 39. No. 1. pp 47–52. 2012.

D. David, J. A. Gansau, and J. O. Abdullah. “Effect of NAA and BAP on protocorm proliferation of Borneo Scented Orchid Vanda helvola”. AsPac J. Mol. Biol. Biotechnol. Vol. 18. No. 1. pp 221-224, 2010.




DOI: http://dx.doi.org/10.18517/ijaseit.8.1.1162

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