Screening and Enzyme Activity of Cellulase-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of the Palm Oil (Elaieis guineensis)

Tri Yuliana, Efri Mardawati, Emilda A. Febrianti, Agus T. Hartono, Souvia Rahimah


Cellulase enzymes have an important role in biocatalyst technology, especially in the pulp industry. Cellulase is synthesized by a large type of microorganisms, including cellulose-producing bacteria isolated from Kemiri Sunan (Reutealis trisperma) shell and empty fruit bunches of the palm oil (EFBPO). In this study, the screening of cellulase-producing bacteria isolated from Kemiri Sunan shell and EFBPO were carried out in order to evaluate its enzyme activity. The method used was an experimental method followed by descriptive analysis using four treatments with two repetitions. This study includes; (1) Lignocellulosic biomass analysis, (2) Isolation of cellulase-producing bacteria, (3) Qualitative assay of cellulase-producing bacteria, (4) Enzyme activity assay. Both cellulose and hemicellulose contents in Kemiri Sunan shell were 27.38% and 44.46%, respectively, and 48.55% and 28.06% in empty fruit bunches of palm oil.  Qualitative assay of cellulase-producing bacteria from Kemiri Sunan shell resulted in the widest clear zone accounting of 77.19% (isolate K2) and 73.06% (isolate T3) from EFBPO. Screening continued by measuring the cell growth with an interval time of 8, 24, 32 and 48 h. The enzyme activity showed the highest cellulase activity (isolate K3) from Kemiri Sunan shell was 4.3 × 10-2 U/mL after 48 h incubation. However, isolate T4 from EFBPO resulted in higher cellulase activity than that Kemiri Sunan shell, accounting of 7.2 × 10-2 U/mL. Our study suggests that cellulase-producing bacteria from Kemiri Sunan and EFBPO has been screened to synthesize cellulase enzyme.


Kemiri Sunan; cellulose; screening; enzyme activity; palm oil.

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Seprianto, “Isolation and screening of cellulolytic bacteria from various types of soil as a producer of cellulase enzymes,†IJOBB, vol. 2, no. 3, 2017.

D. N. Sari, H. Setiyawan, and Abun, “The Effect of Long Fermentation by Bacillus Licheniformis Continued by Saccharomyces Cereviseae On Shrimp Waste On Content of Protein and Glucose Product,†2016.

M. Herman, Pecan Sunan (Reutelis candlenut sunan (Blanco) airy Shaw) Vegetable Oil-Producing Plants and Land Conservation. Niaga Swadaya, 2013.

A. Nadia, Fauziah, and E. Mayori, “Potential of Lignocellulosic Oil Palm Waste in South Kalimantan for Bioethanol and Xylitol Production,†J. Sci. Educ. Innov., vol. 8, pp. 41–51, 2017.

Erwinsyah, A. Afriani, and T. Kardiansyah, “Palm Oil Bunch and Potential Opportunities as Raw Material for Pulp and Paper,†vol. 5, pp. 79–88, 2017.

B. D. Argo and R. Yulianingsih, “Utilization of Cellulase Enzymes from Trichoderma Reseei and Aspergillus Niger as Catalysts for Rice Straw Enzymatic Hydrolysis with Microwave Pretreatment.,†J. Bioproses Komod. Trop., vol. 1(1), pp. 36–43, 2013.

M. Rahman and T. A. Susanti, “Test of Cellulolytic Bacteria Cellulase Activity Derived from Oil Palm Empty Fruit Bunches,†Politekniki Perkebunan Negeri Samarinda, 2016.

A. K. J and O. Omar, “Isolation of Cellulolytic Fungi from Wings - Kinabalu Sabah.,†J. Biodivers. Bio-Century, pp. 1–6, 1988.

E. Coast, V. Jennifer, and G. Thiruneelakandan, Enzymatic Activity of Marine Lactobacillus Species from South. 2015.

J. Perez, “Biodegradation and biological treatments of cellulose , hemicellulose and lignin : an overview,†Int. J. Microbiol., pp. 53–63, 2002.

Darnoko, Potential Use of Palm Oil Lignocellulose Waste Through Bioconversion. Jakarta: Agro Media Pustaka, 1992.

B.C Behera, B.K. Sethi, R.R. Mishra, S.K. Dutta and H.N. Thatoi, “ Microbial cellulases-Diversity and biotechnology with reference to mangrove environtment: A reviewâ€, J.Gen. Eng and Biotech, vol. 15, pp 197-210, 2017.

MaríaCiudad, MuleroVirginiaFernández, R. CruzMatallana, and GonzálezPatriciaMorales., “Dietary fiber sources and human benefits: The case study of cereal and pseudocereals,†Adv. Food Nutr. Res., vol. 90, pp. 83–134, 2019.

F. J. Mc. Donald. RG, Pulp and Paper Manufacture. Bandar Lampung, 1969.

J. Casey, “Pulp and Paper Chemistry and Chemical Technology,†vol. 1, 1980.

A. Taleb, “Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli,†Aust. J. Basic Appl. Sci., vol. 3, 2009.

Subagyo, S., Margino., Triyanto, T., Ari , S., “Effect of pH, Temperature and Salinity in Growth and Organic Acid Production of Lactid Acid Bacteria Isolated from Penaieid shrimp Intestineâ€, Ilmu Kelautan: Indonesian Journal of Marine Sciences, vol 20(4), pp. 187-194, 2017.

Mulyasari, M., Irma, M., MTD Sunarno, “Isolation, Selection and Identification of Cellulolytic Bacteria from Seaweedâ€, J. Riset Akuakultur, vol. 10, 2015.

Y. Kim, H. Jung, and J. Pan, “Bacterial Cell Surface Display of an Enzyme Library for Selective Screening of Improved Cellulase Variants,†J. Appl. Environ. Microbiol., vol. 66, pp. 788–793, 2000.

L. Hankin and S.L. Anagnostakis, “The Use of Solid Media for Detection of Enzyme Production by Fungi,†J. Mycologia., vol. 67, no. 3, pp. 597-607, 1975.

M. Peleg and M. G. Corradini, “Microbial Growth Curves : What the Models Tell Us and What They Cannot,†Crit. Rev. Food Sci. aand Nutr., vol. 51, no. August 2014, pp. 917–945, 2011.

Subagiyo, S., Margino, S., Triyanto, T., & Ari S, “Effects of pH, Temperature and Salinity in Growth and Organic Acid Production of Lactid Acid Bacteria Isolated from Penaeid Shrimp Intestine,†Indon. J. of Marine Sci, vol. 20(4), pp. 147, 2017.

V. Jennifer, E. Coast, and G. Thiruneelakandan, “Enzymatic Activity of Marine Lactobacillus Species from South. Jakarta, 2015.

Sastrohamidjojo, Organic Chemistry, Sterochemistry, Fat, and Protein. Yogyakarta, Indonesia: Gadjah Mada University Press, 2005.

M. I. Jay, “Test of Cellulase Enzyme Activity in Molds from the Pari Islands Waters of the Thousand Islands,†Bogor Agric. Inst., 2014.

Raifah M., Maswati B,â€Identification Termophilic Bacteria from Lejja Hot Spring, Kab. Soppeng†vol.4, pp 31-42, 2016.

S.P. Gautam and P.S. Bundela “ Cellulase Production by Pseudomonas sp. Isolated from Municipal solid waste compost,†Int. J. of Academic Research, vol. 2 no. 6, 2010.

Maratun S.A., Baharuddin, M.,â€Production and cellulose enzyme activity from Bacillus subtilisâ€, JOM FMIPA UIN Alaudin, pp. 78-90, 2015.



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