The Linear Model of Saccharomyces cerevisiae Turbidity in Liquid Media

Ahmad Syauqi, Hari Santoso, Siti Nurul Hasana

Abstract


The study aims to investigate the relationship between the turbidity and density or the total suspended inorganic particles have been obtained many models. The live cells as the homogeneous particle are presumed to cause turbid in liquid media, and that has a linear relationship that can be utilized on the cell counting. The method for the term of clouded liquid form is the measurement based on the reflection and scattered of light, i.e., the turbidimetry. Knowledge attainment of microbial cell counting should be answered how many Nephelometric Turbidity Unit of the one cell. We work to obtain a turbidity model of cells in water-based media for the estimation of cell numbers. This paper aims to construct the computational structure on the turbidity modeling of Saccharomyces cerevisiae in pure water and to test a consistent model in liquid nutrients medium. The modeling was performed in systematized stages of the diagnostic-analysis-test; the regression assurances, the simulation of the lowest error, and the coefficient value itself of turbidity factors. We constructed an optimal analysis and diagnosis to create a computational structure of cell turbidity modeling. The measurement and stopping bivariate elimination of the simulation is a subsystem of the algorithm of obtaining and testing models. The first mathematics model is a standard curve on turbidimetry, and the second, turbidity mathematics model of cell growth in liquid nutrients medium. Both models have an equal coefficient of cell turbidity. The turbidity coefficient of cell growth time interval in the carbonyl diamide - potato dextrose broth is significant.


Keywords


Cell; particle; light; computation; turbidimetry.

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DOI: http://dx.doi.org/10.18517/ijaseit.11.1.10872

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