Characterization of Probiotic Bacterial Candidates from Jatinangor-Indonesia Breast Milk

In-In Hanidah, Indira Lanti Kaya Putri, Wendry Setiyadi Putranto, Bambang Nurhadi, Debby Moody Sumanti

Abstract


Breast milk is an important nutrient for neonates in body’s nutritional needs and immune system formation.  Some research show breast milk generally contains Bifidobacteria and probiotic bacteria species of Lactobacillus. Microbiota in the breast milk from every area is diverse, bacteria isolated from healthy mothers’ breast milk from Taiwan and six regions of China (Central, East, North, Northeast, South, and Southwest China). It shows Streptococcaceae (24.4%), Pseudomonadaceae (14.0%), Staphylococcaceae (12.2%), Lactobacillaceae (6.2%), and Oxalobacteraceae (4.8%). Germany or Austria provided the breast milk from 160 women contain L. salivarius (35.00%), L. fermentum (25.00%), L. gasseri (21.88%), and Bifidobacterial species (13.75%). Spanish   provided Staphylococcus, Pseudomonas, Streptococcus, and Acinetobacter dominated the breast milk from 21 healthy mother. In this study, isolation and characterization of candidates for probiotic bacteria from fifteen breast milk samples from Sumedang – Indonesia. The main purpose of this study was to isolate and identify probiotic bacteria that are resistant pH 2 and tolerance of 0.3% bile concentration. The results showed that were twelve isolate candidate probiotic bacteria able to grow on pH 2 media for 2 hours and tolerance of 0.3% bile concentration. Only two of them had the best growth and potential probiotic candidates. Base on Biochemical identification using the Vitek 2.0 Card type: ANC testing instrument 00001658F4A9 (12903), there are Staphylococcus hominis (8.3%) and Lactobacillus plantarum (8.3%). In the future, S. hominis will be probiotic bacteria that can be applied as functional food.

Keywords


breast milk; probiotic; Staphylococcus hominis; Lactobacillus plantarum; functional food.

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References


K. Le Doare, B. Holder, A. Bassett, and P. S. Pannaraj, “Mother ’ s Milk : A Purposeful Contribution to the Development of the infant Microbiota and immunity,†Front. Immunol., vol. 9, no. February, pp. 1–10, 2018.

R. Martín et al., “Cultivation-independent assessment of the bacterial diversity of breast milk among healthy women,†Res. Microbiol., vol. 158, no. 1, pp. 31–37, 2007.

M. and E. K. E.-H. Witkowska-zimny, “Cells of human breast milk,†Cell. Mol. Biol. Lett., pp. 1–11, 2017.

A.-U. B. . Nino, Serrano J.C., Solis-Pacheco J.R., Gutierrez-Padilla J.A, Cobián-García, Cavazos-Garduño A, González-Reynoso O., “Isolation and Identification of Lactic Acid Bacteria from Human Milk with Potential Probiotic Role,†J. Food Nutr. Res., vol. 4, no. 3, pp. 170–177, 2016.

R. Martín et al., “Isolation of bifidobacteria from breast milk and assessment of the bifidobacterial population by PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR,†Appl. Environ. Microbiol., vol. 75, no. 4, pp. 965–969, 2009.

A. Soto, V. Martín, E. Jiménez, I. Mader, J. M. Rodríguez, and L. Fernández, “Lactobacilli and Bifidobacteria in Human Breast Milk : Influence of Antibiotherapy and Other Host and Clinical Factors,†J. Pediatr. Gastroenterol. Nutr., vol. 59, no. 1, pp. 78–88, 2014.

R. Martín, M. Olivares, M. L. Marín, L. Fernández, J. Xaus, and J. M. Rodríguez, “Probiotic potential of 3 Lactobacilli strains isolated from breast milk.,†J. Hum. Lact., vol. 21, no. 1, pp. 8-17; quiz 18-21, 41, 2005.

S. Li et al., “Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China,†Front. Microbiol., vol. 8, no. 965, pp. 1–15, 2017.

A. Boix-amorós, M. C. Collado, and A. Mira, “Relationship between Milk Microbiota , Bacterial Load , Macronutrients , and Human Cells during Lactation,†Front. Microbiol., vol. 7, no. April, pp. 1–9, 2016.

M. M. Esther Jimenez, Javier de Andres, “Metagenomic Analysis of Milk of Healthy and Mastitis-Suffering Women,†J. Hum. Lact., pp. 10–12, 2015.

H. Yavuzdurmaz, “Determination of Probiotic Properties of Lactic Acid Bacteria From Human,†Izmir Institute of Technology, 2007.

D. Andrian, D. Rizkinata, T. Steven, R. Susanto, J. Lucy, and T. T. Jan, “Isolation of Streptococcus thermophilus and Lactobacillus delbrueckii as Starter Culture Candidate Originated from Indonesian Cow ’ s Milk,†Microbiol. Biotechnol. Lett., vol. 46, no. 3, pp. 201–209, 2018.

R. Kunchala, R. Banerjee, S. D. Mazumdar, and P. Durgalla, “Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics,†Arican J. Biotechnol., vol. 15, no. 16, pp. 613–621, 2016.

C. Orsi and A. V. Zambrini, “Probiotics and Prebiotics,†Adv. Dairy Prod., vol. 8, no. 6, pp. 37–57, 2017.

H. K. Sahadeva, R.P.K., Leong, S.F., Chua, K.H., Tan, C.H., Chan, H.Y., Tong, E.V., Wong, S.Y.W., and Chan, “Survival of commercial probiotic strains to pH and bile,†Int. Food Res. J., vol. 18, no. 4, pp. 1515–1522, 2011.

C. N. Jacobsen, V. R. Nielsen, A. E. Hayford, and P. L. Møller, “Screening of Probiotic Activities of Forty-Seven Strains of Lactobacillus spp . by In Vitro Techniques and Evaluation of the Colonization Ability of Five Selected Strains in Humans,†Appl. Environ. Microbiol., vol. 65, no. 11, pp. 4949–4956, 1999.

S. J. Mannan, R. Rezwan, S. Rahman, and K. Begum, “Isolation and Biochemical Characterization of Lactobacillus species from Yogurt and Cheese samples in Dhaka Metropolitan Area,†Bangladesh Pharm. J., vol. 20, no. 1, pp. 27–33, 2017.

S. Hossain, A. Al-bari, M. Imam, and I. Wahed, “Biochemical Characterization of Probiotics Available in Bangladesh,†J. Sci. Res., vol. 1, no. January, pp. 101–108, 2016.

C. I. Ayo-Olalusi, “Isolation and Identification of Probiotics Pediococcus pentosaceus2 and Pediococcus pentosaceus1 from the Gut of Tilapia Guineensis for Use in Aquaculture Production,†Int. J. Res. Stud. Microbiol. Biotechnol., vol. 3, no. 1, pp. 15–20, 2017.

M. Z. Hoque, F. Akter, K. M. Hossain, M. S. M. Rahman, M. M. Billah, and K. M. D. Islam, “Isolation , Identification and Analysis of Probiotic Properties of Lactobacillus Spp . From Selective Regional Yoghurts,†World J. Dairy Food Sci., vol. 5, no. 1, pp. 39–46, 2010.

C. Sung, B. Kim, S. Kim, H. Joo, and P. I. Kim, “Probiotic potential of Staphylococcus hominis MBBL 2 – 9 as anti- Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman,†J. Appl. Microbiol., vol. 108, pp. 908–916, 2010.

L. Chou and B. Weimer, “Isolation and Characterization of Acid- and Bile-Tolerant Isolates from Strains of Lactobacillus acidophilus 1,†J. Dairy Sci., vol. 82, no. 1, pp. 23–31, 1999.

P. Datta, S. Banerjee, A. Naha, S. Pal, J. Konar, and C. Sahu, “Staphylococcus Hominis Subsp . Hominis Causing Non-Lactational Breast Abscess: A Rare Pathogen Reported,†J. Evol. Med. Dent. Sci., vol. 4, no. 16, pp. 2830–2832, 2015.

A. Khusro, C. Aarti, A. Dusthackeer, and P. Agastian, “Anti-tubercular and probiotic properties of coagulase-negative staphylococci isolated from Koozh , a traditional fermented food of South India,†Microb. Pathog., vol. 114, no. 2018, pp. 239–250, 2017.




DOI: http://dx.doi.org/10.18517/ijaseit.9.5.10124

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